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Reactive Labels

Square, Seta, and SeTau dyes are a series of dyes that span the whole visible spectrum and the NIR. They are available with various functionalities: carboxylic acids, as amine-reactive NHS esters for labeling of amino groups on peptides, proteins or amino-modified DNA, as maleimides for coupling to thiol-groups in proteins and thiol-modified oligos and as amino-modified labels (on request).

With a few exceptions all these reagents are available in either 1 mg or 5 mg pack sizes. Please do inquire about the availability of bulk quantities.

Excitation Light Sources Characteristics Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Medium λ abs
[nm]
ε
[M–1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                               
K1-204
K1-204
SeTau-380-NHS
SeTau-380-NHS
NH2 Water 270 23800 480 56 32.5 BSA, D/P=1 383 23800 468 44 30.6
K5-3222
K5-3222
Seta-580-NHS
Seta-580-NHS
NH2 PB 7.4 578 90000 602 55 BSA, D/P=1 585 609 15
K7-547
K7-547
SeTau-405-NHS
SeTau-405-NHS
NH2 PB 7.4 405 13800 518 80 9.3 IgG, D/P=8 405 13800 518 19 8.4
K7-567
K7-567
SeTau-405-Azide
SeTau-405-Azide
triple-CC PB 7.4 405 13800 518 80 9.3 EtOH 391 15000 498 55 8.5
K8-1342
K8-1342
Seta-670-NHS
Seta-670-NHS
NH2 PB 7.4 667 180000 688 7 0.42 BSA, D/P=1.5 681 180000 696 27 2.43
K7-545
K7-545
SeTau-425-NHS
SeTau-425-NHS
NH2 PB 7.4 425 4200 545 39 26.2 BSA, D/P=1 427 4200 528 16 26.2
K8-1346
K8-1346
Seta-670-Azide
Seta-670-Azide
triple-CC PB 7.4 667 180000 686 7 PB 7.4 667 180000 686 7
K8-1352
K8-1352
Square-660-NHS
Square-660-NHS
NH2 PB 7.4 657 182000 676 3 0.27 BSA D/P=1.2 676 182000 695 13 3.32
K8-1357
K8-1357
Square-680-NHS
Square-680-NHS
NH2 MeOH 668 208000 690 10 CHCl3 683 202000 701 47
K8-1367
K8-1367
Seta-670-pH-di-NHS
Seta-670-pH-di-NHS
NH2 PB 6.0 672 93000 694 19 IgG, D/P=1 684 703 7
K8-1384
K8-1384
Seta-700-NHS
Seta-700-NHS
NH2 PB 7.4 687 177000 703 6 IgG D/P=1 705 177000 715 4
K8-1407
K8-1407
Square-650-pH-NHS
Square-650-pH-NHS
NH2 PB 5.6 653 135000 671 16 1.17 IgG, D/P=0.8
pH=2.0
662 135000 677 7 1.52
K8-1626
K8-1626
Seta-633-di-NHS
Seta-633-di-NHS
NH2 PB 7.4 635 192000 644 12 0.7 IgG, D/P=1 639 192000 650 33 3
K8-1641
K8-1641
Seta-632-Maleimide
Seta-632-Maleimide
SH PB 7.4 633 270000 642 5
K8-1642
K8-1642
Seta-632-NHS
Seta-632-NHS
NH2 PB 7.4 632 280000 641 6 IgG, D/P=1 637 647 18
K8-1649
K8-1649
SQ-740-NHS
SQ-740-NHS
NH2 PB 7.4 740 44000 0 0
K8-1663
K8-1663
Seta-633-NHS
Seta-633-NHS
NH2 PB 7.4 633 250000 644 7 0.25 IgG D/P=1 637 250000 647 26 1.49
K8-1667
K8-1667
Seta-635-pH-di-NHS
Seta-635-pH-di-NHS
NH2 PB 7.4 640 188000 656 33 1.8 IgG, D/P=1 646 663 21
K8-1672
K8-1672
Seta-646-NHS
Seta-646-NHS
NH2 PB 7.4 646 207000 656 10 0.38 IgG, D/P=1 650 207000 661 33
K8-1682
K8-1682
Seta-660-di-NHS
Seta-660-di-NHS
NH2 PB 7.4 661 220000 672 11 0.9 IgG D/P=1 665 220000 675 49
K8-1696
K8-1696
Seta-633-Azide
Seta-633-Azide
triple-CC PB 7.4 633 250000 644 7 PB 7.4 633 250000 644 7
K8-1902
K8-1902
SQ-565-NHS
SQ-565-NHS
NH2 Water 560 22000 0 0 CHCl3 565
K8-1922
K8-1922
SQ-780-NHS
SQ-780-NHS
NH2 MeOH 782 115000 0 0 CHCl3 806 0 0
K8-3002
K8-3002 new
Seta-470-NHS
Seta-470-NHS
NH2 water 469 50000 521 1.9 BSA, D/P=1 469 515 28
K8-3335
K8-3335
Seta-555-NHS
Seta-555-NHS
NH2 PB 7.4 555 155000 570 7 IgG, D/P=1 556 571 13
K8-3345
K8-3345
Seta-555-DBCO
Seta-555-DBCO
N3 PB 7.4 555 155000 570 7
K8-3346
K8-3346
Seta-555-Azide
Seta-555-Azide
triple-CC PB 7.4 555 155000 570 7
K8-5035
K8-5035
Seta-650-NHS
Seta-650-NHS
NH2 PB 7.4 651 200000 671 28 IgG, D/P=1 654 673 37
K8-5036
K8-5036
Seta-650-Maleimide
Seta-650-Maleimide
SH PB 7.4 652 200000 672 28
K8-5045
K8-5045
Seta-650-DBCO
Seta-650-DBCO
N3 PB 7.4 653 200000 674 28
K8-5046
K8-5046
Seta-650-Azide
Seta-650-Azide
triple-CC PB 7.4 651 200000 671 28
K8-5075
K8-5075
Seta-680-NHS
Seta-680-NHS
NH2 PB 7.4 678 200000 698 24 IgG, D/P=1 680 701 30
K8-7045
K8-7045
Seta-750-DBCO
Seta-750-DBCO
N3 PB 7.4 754 234000 782 21
K8-7046
K8-7046
Seta-750-Azide
Seta-750-Azide
triple-CC PB 7.4 750 200000 779 22
K8-7055
K8-7055
Seta-780-NHS
Seta-780-NHS
NH2 PB 7.4 777 215000 807 1.2
K9-4119
K9-4119 new
SeTau-665-NHS
SeTau-665-NHS
NH2 PB 7.4 664 160000 712 53 3.1 IgG, D/P=1.5 662 716 46
K9-4142
K9-4142
SeTau-647-di-NHS
SeTau-647-di-NHS
NH2 PB 7.4 650 200000 694 65 3.2 IgG, D/P=0.5 649 200000 691 58 3.2
K9-4148
K9-4148
SeTau-647-Maleimide
SeTau-647-Maleimide
SH PB 7.4 648 200000 692 45 3.2 PB 7.4 648 200000 692 45 3.2
K9-4149
K9-4149
SeTau-647-NHS
SeTau-647-NHS
NH2 PB 7.4 649 200000 695 61 3.2 IgG, D/P=1 648 200000 694 59 3.3
K9-4169
K9-4169 new
SeTau-670-NHS
SeTau-670-NHS
NH2 PB 7.4 673 275000 694 36 1.6 IgG D/P = 4 672 693 14 1.75
K8-3402
K8-3402
Zy3-NHS
Zy3-NHS
NH2 PB 7.4 549 150000 564 4 0.3
K8-5402
K8-5402
Zy5-NHS
Zy5-NHS
NH2 PB 7.4 647 250000 665 27 1
K8-5422
K8-5422
Zy5.5-NHS
Zy5.5-NHS
NH2 PB 7.4 676 190000 694 23 1
K8-3403
K8-3403
Zy3-Maleimide
Zy3-Maleimide
SH PB 7.4 549 150000 564 4 0.3
K8-5403
K8-5403
Zy5-Maleimide
Zy5-Maleimide
SH PB 7.4 647 250000 665 27 1
K8-5423
K8-5423
Zy5.5-Maleimide
Zy5.5-Maleimide
SH PB 7.4 676 190000 694 23 1
K8-1388
K8-1388
Seta-700-di-NHS
Seta-700-di-NHS
NH2 PB 7.4 688 180000 704 11 IgG D/P=1 696 713 23
K8-1341
K8-1341
Seta-670-Maleimide
Seta-670-Maleimide
SH PB 7.4 667 180000 688 7
K8-1671
K8-1671
Seta-646-Maleimide
Seta-646-Maleimide
SH PB 7.4 647 210000 657 8 IgG, D/P=1 650 661 31
K4-205
K4-205 new
Seta-375-NHS
Seta-375-NHS
NH2 PB 7.4 372 24500 476 86 3.1 IgG, D/P=5 372 24500 480 42 3.5
K7-548
K7-548
SeTau-405-Maleimide
SeTau-405-Maleimide
SH PB 7.4 405 13800 518 51 9.1 EtOH 391 15000 498 32 8.5
K8-7522
K8-7522
Seta-750-NHS
Seta-750-NHS
NH2 PB 7.4 753 230000 780 14 IgG D/P=1 756 783 17
KS-650
KS-650
590–650 nm FLT Standard
590–650 nm FLT Standard
PB 7.4 647 250000 665 27 1.05
K8-3003
K8-3003 new
Seta-470-Maleimide
Seta-470-Maleimide
SH Water 469 50000 521 1.9 EtOH 462 50000 515 2.2
K8-7402
K8-7402
Zy7-NHS
Zy7-NHS
NH2 PB 7.4 749 200000 774 13 0.57
DD1
DD1
DD1 2D DIGE Kit
DD1 2D DIGE Kit
NH2
DD2
DD2
DD2 2D DIGE Kit
DD2 2D DIGE Kit
NH2
DD3
DD3
DD3 2D DIGE Kit
DD3 2D DIGE Kit
NH2
K7-544
K7-544
SeTau-425-Maleimide
SeTau-425-Maleimide
SH PB 7.4 425 4200 545 39 26.2 PB 7.4 425 4200 545 39 26.2
K4-225
K4-225 new
Seta-385-NHS
Seta-385-NHS
NH2 PB 7.4 384 25000 512 78 IgG, D/P=6 387 25000 505 29
K8-1389
K8-1389 new
Seta-700-NHS
Seta-700-NHS
NH2 PB 7.4 688 162000 703 14 IgG D/P=1 694 710 18
K6-5052
K6-5052 new
Seta-405 NHS
Seta-405 NHS
NH2 PB 7.4 401 30000 452 IgG D/P=1 405 553 56
K4-215
K4-215 new
Seta-400-NHS
Seta-400-NHS
NH2 PB 7.4 399 24000 576 34 IgG D/P=1 401 569 19
K9-4179
K9-4179 new
SeTau-680-NHS
SeTau-680-NHS
NH2 PB 7.4 683 215000 705 58 2.9 IgG D/P=1 682 703 47

 

Labeling of Amino-groups:

The following functional groups available for labeling of amino-groups in proteins or amino-modified oligos:

Labeling of amino-modified biomolecules

Among all these functionalities N-hydroxysuccinimide (NHS) ester are the preferred reactive functionality for labeling of amino-groups due to the formation of highly stable amide bonds with amines. Sulfonyl chlorides also form stable sulfamide bonds but the reaction also generates HCl, which is a not so desirable by-product. In addition sulfonyl chlorides are more difficult to synthesize.

 

Labelling of Thiol-groups:

Maleimides are the preferred functionality for labeling of small molecules such as peptide and oligo-nucleotides. Maleimides form bonds with thiols at relatively mild conditions and with reasonable yields. Maleimides are less susceptible to hydrolysis in comparison with NHS esters.

We would like to refer you to our labeling procedures for specific guidance on how to label with NHS-esters or maleimides.

Labeling of thiol-modified biomolecules

 

Azides:

Several of our most sensitive Seta Dyes are now available as azides to be reacted with C≡C-triple bonds in either a Cu(I)-catalized or Cu-free 1,3-dipolar cycloaddition reaction to a triazole (see figure). Both reactions proceed in aqueous environments with adequate efficiency.

Cu-catalyzed cycloaddition:

Cu-catalyzed cycloaddition

 

Cyclo-Octyne (DBCO) Derivatives:

Strain-mediated cyclization reactions of cyclo-octynes proceed without the need for a Cu-catalyst. The metal-free reaction has wider applicability and is compatible with cell-based applications.

Cu-free, strain-mediated cycloaddition:

Cu-free, strain-mediated cycloaddition

 

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