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Product Name : Seta-470-NHS

Product Number:
K8-3002
Target Group:
NH2
Excitation Light Sources:
Medium 1:
water
λAb max. [nm]:
469
ε [M–1 cm–1]:
50000
λEm max. [nm]:
521
QY [%]:
1.9
Medium 2:
BSA, D/P=1
λAb max. [nm]:
469
λEm max. [nm]:
515
QY [%]:
28
Medium 3:
BSA, D/P=1
λAb max. [nm]:
469
ε [M–1 cm–1]:
50000
λEm max. [nm]:
515
QY [%]:
28
Medium 4:
EtOH
λAb max. [nm]:
462
λEm max. [nm]:
515
QY [%]:
2.2
Specs Sheet:
Download

Description

  • Highly hydrophilic, viscosity-sensitive, amine-reactive label containing one NHS-ester group

 

Applications

  • Assessment of viscosity of probe environment — see [31]
  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
  • Viscosity sensitive fluorescence lifetime label — this label exhibits a distinct lifetime change upon changing the viscosity of the environment

 

Advantages

  • Perfectly suited for excitation with the 330, 436, and 470-nm diode lasers and UV light
  • Large Stokes' shift
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility; this label does not alter the solubility of the protein conjugate 
  • High photostability