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Product Number: K8-7055

Product Name:
Seta-780-NHS
Target Group:
NH2
Excitation Light Sources:
700 750 780
Medium 1:
PB 7.4
λAb max. [nm]:
777
ε [M–1 cm–1]:
215000
λEm max. [nm]:
807
QY [%]:
1.2
Specs Sheet:
Download

Description

  • Seta-780-NHS (K8-7055) is a hydrophilic, amine-reactive label containing one NHS-ester group with almost identical absorption and emission as ICG and can therefore be used with these filter sets. 

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
  • Fluorescence intensity and fluorescence polarization-based applications
  • Resonance Energy Transfer (RET)
  • Flow Cytometry
  • Immunofluorescence
  • Gene Expression
  • Homogeneous Assays
  • Microarrays

 

Advantages

  • Perfectly suited for excitation with the 700 nm, 750 nm or 780 nm diode lasers
  • Sensitive; high extinction coefficients and the quantum yield in water is as much as twice higher compared to Indocyanine green (ICG)
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility; this label does not alter the solubility of bioconjugates 
  • High photostability
  • Low molecular weight  — Seta-780 does not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides and amino-modified lipids