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Product Number: K8-1682

Product Name:
Seta-660-di-NHS
Target Group:
NH2
Excitation Light Sources:
635 650
Medium 1:
PB 7.4
λAb max. [nm]:
661
ε [M–1 cm–1]:
220000
λEm max. [nm]:
672
QY [%]:
11
FLT [ns]:
0.9
Medium 2:
IgG D/P=1
λAb max. [nm]:
665
ε [M–1 cm–1]:
220000
λEm max. [nm]:
675
QY [%]:
49
Specs Sheet:
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Description

  • Extremely bright, hydrophilic, amine-reactive label containing two NHS-ester groups. QYs of up to 50% when covalently labeled to IgG.

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
  • Fluorescence intensity and fluorescence polarization-based applications
  • Resonance Energy Transfer (RET)
  • Flow Cytometry
  • Immunofluorescence
  • Gene Expression
  • Homogeneous Assays
  • Microarrays

 

Advantages

  • Perfectly suited for excitation with the 635-nm and 650-nm diode lasers
  • Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules
  • Brighter replacement for Alexa 660 (QY = 37%, ε = 132,000 M–1cm–1). Seta-660: QY = 49%, ε = 220,000 M–1cm–1
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility; this label does not alter the solubility of the bioconjugate 
  • High photostability; e.g. compared to fluorescein, Cy5 and Alexa 647 or Alexa 660
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides and amino-modified lipids