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Product Name : SeTau-405-Azide

Product Number:
K7-567
Target Group:
triple-CC
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
405
ε [M–1 cm–1]:
13800
λEm max. [nm]:
518
QY [%]:
80
FLT [ns]:
9.3
Medium 2:
EtOH
λAb max. [nm]:
391
ε [M–1 cm–1]:
15000
λEm max. [nm]:
498
QY [%]:
55
FLT [ns]:
8.5
Specs Sheet:
Download

Description

  • Hydrophilic, alkyne-reactive, long-lifetime reagent for click chemistry containing one azide function. Azides react with C≡C triple bonds in either a Cu(I)-catalyzed or Cu-free 1,3-dipolar cycloaddition reaction to triazoles. The dye contains a single positive charge and chloride as the counter-ion.

 

 

Applications

  • Click Chemistry reagent.
  • Fluorescence lifetime assays.
  • Fluorescence polarization-based assays of high molecular weight antigens.

 

Advantages

  • Highly fluorescent label for proteins and oligos.
  • High quantum yield (Q.Y.) ~ 80 % (water).
  • Long fluorescence lifetime of 9 ns in water.
  • Perfectly suited for excitation with the 380-nm and 404-nm diode lasers.
  • Large Stokes' shift : > 100 nm.
  • High fundamental polarization P0 = 475 mP.
  • Highly soluble in aqueous buffer (500 mg/L at 20 °C)