Tandem Conjugates

Phycobiliprotein-tandem-conjugates permit the simultaneous detection of several parameters on cell surfaces using the 405 nm, 488 nm or 633 nm laser lines. We refer you to Flow Cytometry for a list of common fluorescently labeled phycobiliproteins tandem dyes that are frequently used in Flow Cytometry including a list of our superior Seta dye and Seta tandem dye replacements.

SETA BioMedicals has spent several years in research to produce and optimize these phycobiliprotein-tandems. These ready-made tandems of R-PE, PerCP and APC are optimized for brightness, spill-over and photostability. See specs sheets below for more detailed information.

 

Excitation Light Sources Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                 
K10-6801
K10-6801 new
Seta-PerCP-680
Seta-PerCP-680
PB 7.4 481 380,000 676
K10-7801
K10-7801 new
Seta-APC-780
Seta-APC-780
PB 7.4 651 1,100,000 780
K10-6701
K10-6701 new
Seta-R-PE-670
Seta-R-PE-670
PB 7.4 488 1,100,000 671
K10-0775
K10-0775 new
Seta-R-PE-775
Seta-R-PE-775
PB 7.4 488 1,100,000 774
K10-7051
K10-7051 new
Seta-APC-705
Seta-APC-705
PB 7.4 655 1,100,000 703

 

The table below shows a listing of our proprietary, superior replacements for the most common tandems currently used in flow cytometry: 

 

Laser Source Common Tandems Used Superior Seta Replacements

488 nm

R-PE-Texas Red coming soon!
R-PE-Cy5 Seta-R-PE-670
 PerCP-Cy5.5, R-PE-Cy5.5 Seta-PerCP-680
R-PE-Cy7 Seta-R-PE-775

633 nm

 APC-AF700 Seta-APC-705
APC-Cy7, APC-H7, APC-AF750 Seta-APC-780

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