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Product Name : Seta-385-NHS

Product Number:
K4-225
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
384
ε [M–1 cm–1]:
25000
λEm max. [nm]:
512
QY [%]:
78
Medium 2:
IgG, D/P=6
λAb max. [nm]:
387
ε [M–1 cm–1]:
25000
λEm max. [nm]:
505
QY [%]:
29
Specs Sheet:
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Description

  • Hydrophilic, positively charged, amine-reactive label containing one NHS-ester group

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
  • Resonance Energy Transfer (RET)
  • Flow Cytometry
  • Immunofluorescence
  • Gene Expression
  • Homogeneous Assays
  • Assessment of protein structure

 

Advantages

  • Perfectly suited for excitation with the 330 and 380-nm diode lasers and UV light
  • Extremely large Stokes' shift of over 120 nm 
  • High extinction coefficients and high quantum yields of 78% 
  • Low non-specific binding 
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility; this label does not alter the solubility of the protein conjugate 
  • High photostability; e.g. compared to fluorescein
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides