tech1
$0.00

Product Number: K8-1352

Product Name:
Square-660-NHS
Target Group:
NH2
Excitation Light Sources:
380 405 635 650
Medium 1:
PB 7.4
λAb max. [nm]:
657
ε [M–1 cm–1]:
182000
λEm max. [nm]:
676
QY [%]:
3
FLT [ns]:
0.27
Medium 2:
BSA D/P=1.2
λAb max. [nm]:
676
ε [M–1 cm–1]:
182000
λEm max. [nm]:
695
QY [%]:
13
FLT [ns]:
3.32
Specs Sheet:
Download

Description

  • Amine-reactive, lifetime-sensitive fluorescent label containing one reactive NHS-ester group

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
  • Fluorescence polarization assays
  • Homogeneous lifetime-based assays
  • FRET based applications
  • Flow Cytometry

 

Advantages

  • Perfectly suited for excitation with the 670-nm diode laser, 404-nm diode laser, and UV light
  • Sensitive; high extinction coefficients and high quantum yields up to 25% after covalent attachment to proteins and amino-modified oligonucleotides.
  • Low non-specific binding
  • Lifetime sensitive label: fluorescence lifetime will change 10-fold upon binding to an oligonucleotide
  • pH-insensitive between pH 3 and pH 10
  • High photostability; e.g. compared to fluorescein or Cy5TM
  • Low molecular weight  — Square dyes do not add substantial mass to the conjugates
  • Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides