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Product Name : Square-680-NHS

Product Number:
K8-1357
Target Group:
NH2
Excitation Light Sources:
Medium 1:
MeOH
λAb max. [nm]:
668
ε [M–1 cm–1]:
208000
λEm max. [nm]:
690
QY [%]:
10
Medium 2:
CHCl3
λAb max. [nm]:
683
ε [M–1 cm–1]:
202000
λEm max. [nm]:
701
QY [%]:
47
Medium 3:
PB 7.4
λAb max. [nm]:
654
ε [M–1 cm–1]:
138000
λEm max. [nm]:
670
QY [%]:
1
Specs Sheet:
Download

Description

  • Hydrophobic, amine-reactive fluorescent label containing one reactive NHS-ester group.

 

Applications

  • Covalent labeling of amino-modified lipids and oligonucleotides
  • Fluorescence Resonance Energy Transfer (FRET) applications
  • Homogeneous Assays

 

Advantages

  • Perfectly suited for excitation with the 380, 404, 635 and 670-nm diode lasers and UV light
  • Sensitive; high extinction coefficients and quantum yields of protein conjugates
  • Quantum yields increase upon covalent association with biomolecules
  • pH-insensitive between pH 3 and pH 10 
  • High photostability; e.g. compared to fluorescein or Cy5TM
  • Low molecular weight  — Square dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of amino-modified amino-modified lipids