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Product Number: K8-1389

Product Name:
Seta-700-NHS
Target Group:
NH2
Excitation Light Sources:
405 650 680 700
Medium 1:
PB 7.4
λAb max. [nm]:
688
ε [M–1 cm–1]:
162000
λEm max. [nm]:
703
QY [%]:
14
Medium 2:
IgG D/P=1
λAb max. [nm]:
694
λEm max. [nm]:
710
QY [%]:
18
Specs Sheet:
Download

Description

  • Water soluble, amine-reactive fluorescent label with one reactive NHS-ester groups.
  • High extinction coefficient and quantum yields up to 18% when labeled to IgG.

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides.
  • Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule.
  • Resonance Energy Transfer (RET).
  • Flow Cytometry.
  • Immunofluorescence.
  • Gene Expression.
  • Homogeneous Assays.
  • Assessment of protein structure.

 

Advantages

  • Perfectly suited for excitation with the 405, 670 or 690-nm diode lasers
  • Q.Y.s up to 18% upon covalent attachment to IgG
  • pH-insensitive between pH 4 and pH 9
  • Good aqueous solubility; this label does not alter the solubility of the protein conjugate 
  • Higher photostability compared to fluorescein,  Cy7TM or Alexa FluorTM 700
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides