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Immunofluorescence

Immunofluorescence uses fluorescently labeled antibodies to measure antigens or visualize epitopes of interest on cell surfaces. Primary or secondary antibodies can be utilized for these applications: a primary antibody is used against the antigen of interest directly while the secondary antibody couples to a primary antibody.


SETA BioMedicals offers a large number of superior fluorescent labels for immunofluorescence applications. Seta-labels yield in general fluorescent antibodies that are much brighter and more photostable compared to other dyes. Fluorescent labels and tandems for labeling of CD antibodies are provided in the flow cytometry section. Please visit our website for more information or call us at 217 417 2160.

Excitation Light Sources Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                   
K1-204
K1-204
SeTau-380-NHS
SeTau-380-NHS
NH2 Water 270 23800 480 56 32.5
K7-547
K7-547
SeTau-405-NHS
SeTau-405-NHS
NH2 PB 7.4 405 13800 518 80 9.3
K7-545
K7-545
SeTau-425-NHS
SeTau-425-NHS
NH2 PB 7.4 425 4200 545 39 26.2
K8-1352
K8-1352
Square-660-NHS
Square-660-NHS
NH2 PB 7.4 657 182000 676 3 0.27
K8-1626
K8-1626
Seta-633-di-NHS
Seta-633-di-NHS
NH2 PB 7.4 635 192000 644 12 0.7
K8-1641
K8-1641
Seta-632-Maleimide
Seta-632-Maleimide
SH PB 7.4 633 270000 642 5
K8-1642
K8-1642
Seta-632-NHS
Seta-632-NHS
NH2 PB 7.4 632 280000 641 6
K8-1649
K8-1649
SQ-740-NHS
SQ-740-NHS
NH2 PB 7.4 740 44000 0 0
K8-1663
K8-1663
Seta-633-NHS
Seta-633-NHS
NH2 PB 7.4 633 250000 644 7 0.25
K8-1672
K8-1672
Seta-646-NHS
Seta-646-NHS
NH2 PB 7.4 646 207000 656 10 0.38
K8-1682
K8-1682
Seta-660-di-NHS
Seta-660-di-NHS
NH2 PB 7.4 661 220000 672 11 0.9
K8-1902
K8-1902
SQ-565-NHS
SQ-565-NHS
NH2 Water 560 22000 0 0
K8-1922
K8-1922
SQ-780-NHS
SQ-780-NHS
NH2 MeOH 782 115000 0 0
K8-3335
K8-3335
Seta-555-NHS
Seta-555-NHS
NH2 PB 7.4 555 155000 570 7
K8-5035
K8-5035
Seta-650-NHS
Seta-650-NHS
NH2 PB 7.4 651 200000 671 28
K8-5036
K8-5036
Seta-650-Maleimide
Seta-650-Maleimide
SH PB 7.4 652 200000 672 28
K8-7046
K8-7046
Seta-750-Azide
Seta-750-Azide
triple-CC PB 7.4 750 200000 779 22
K8-7055
K8-7055
Seta-780-NHS
Seta-780-NHS
NH2 PB 7.4 777 215000 807 1.2
K9-4119
K9-4119 new
SeTau-665-NHS
SeTau-665-NHS
NH2 PB 7.4 664 160000 712 53 3.1
K9-4142
K9-4142
SeTau-647-di-NHS
SeTau-647-di-NHS
NH2 PB 7.4 650 200000 694 65 3.2
K9-4148
K9-4148
SeTau-647-Maleimide
SeTau-647-Maleimide
SH PB 7.4 648 200000 692 45 3.2
K9-4149
K9-4149
SeTau-647-NHS
SeTau-647-NHS
NH2 PB 7.4 649 200000 695 61 3.2
K8-3402
K8-3402
Zy3-NHS
Zy3-NHS
NH2 PB 7.4 549 150000 564 4 0.3
K8-5402
K8-5402
Zy5-NHS
Zy5-NHS
NH2 PB 7.4 647 250000 665 27 1
K8-5422
K8-5422
Zy5.5-NHS
Zy5.5-NHS
NH2 PB 7.4 676 190000 694 23 1
K8-3403
K8-3403
Zy3-Maleimide
Zy3-Maleimide
SH PB 7.4 549 150000 564 4 0.3
K8-5403
K8-5403
Zy5-Maleimide
Zy5-Maleimide
SH PB 7.4 647 250000 665 27 1
K8-5423
K8-5423
Zy5.5-Maleimide
Zy5.5-Maleimide
SH PB 7.4 676 190000 694 23 1
K8-1388
K8-1388
Seta-700-di-NHS
Seta-700-di-NHS
NH2 PB 7.4 688 180000 704 11
K8-1341
K8-1341
Seta-670-Maleimide
Seta-670-Maleimide
SH PB 7.4 667 180000 688 7
K8-1671
K8-1671
Seta-646-Maleimide
Seta-646-Maleimide
SH PB 7.4 647 210000 657 8
K4-205
K4-205 new
Seta-375-NHS
Seta-375-NHS
NH2 PB 7.4 372 24500 476 86 3.1
K7-548
K7-548
SeTau-405-Maleimide
SeTau-405-Maleimide
SH PB 7.4 405 13800 518 51 9.1
K10-6801
K10-6801 new
Seta-PerCP-680
Seta-PerCP-680
PB 7.4 481 380000 676
K10-7801
K10-7801 new
Seta-APC-780
Seta-APC-780
PB 7.4 651 1000000 780
K10-6701
K10-6701 new
Seta-R-PE-670
Seta-R-PE-670
PB 7.4 488 1100000 671
K8-7522
K8-7522
Seta-750-NHS
Seta-750-NHS
NH2 PB 7.4 753 230000 780 14
K8-3003
K8-3003 new
Seta-470-Maleimide
Seta-470-Maleimide
SH Water 469 50000 521 1.9
K8-7402
K8-7402
Zy7-NHS
Zy7-NHS
NH2 PB 7.4 749 200000 774 13 0.57
K10-0775
K10-0775 new
Seta-R-PE-775
Seta-R-PE-775
PB 7.4 488 1100000 774
K10-7151
K10-7151 new
Seta-APC-715
Seta-APC-715
PB 7.4 651 600000 713
K4-225
K4-225 new
Seta-385-NHS
Seta-385-NHS
NH2 PB 7.4 384 25000 512 78
K6-5052
K6-5052 new
Seta-405 NHS
Seta-405 NHS
NH2 PB 7.4 401 30000 452
K4-215
K4-215 new
Seta-400-NHS
Seta-400-NHS
NH2 PB 7.4 399 24000 576 34
K9-4179
K9-4179 new
SeTau-680-NHS
SeTau-680-NHS
NH2 PB 7.4 683 215000 705 58 2.9

 

 

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