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Product Name : Seta-700-di-NHS

Product Number:
K8-1388
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
688
ε [M–1 cm–1]:
180000
λEm max. [nm]:
704
QY [%]:
11
Medium 2:
IgG D/P=1
λAb max. [nm]:
696
λEm max. [nm]:
713
QY [%]:
23
Medium 3:
MeOH
λAb max. [nm]:
701
ε [M–1 cm–1]:
175000
λEm max. [nm]:
719
QY [%]:
18
Specs Sheet:
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Description

  • Bright, water soluble, amine-reactive fluorescent label containing two reactive NHS-ester groups with. Quantum yields up to 25% when labeled to IgG.

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides.
  • Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule.
  • Resonance Energy Transfer (RET).
  • Flow Cytometry.
  • Immunofluorescence.
  • Gene Expression.
  • Homogeneous Assays.
  • Assessment of protein structure.

 

Advantages

  • Perfectly suited for excitation with the 380, 405, 650, and 670-nm diode lasers, and UV light
  • Q.Y.s up to 25% upon covalent attachment to IgG
  • pH-insensitive between pH 4 and pH 9
  • Good aqueous solubility; this label does not alter the solubility of the protein conjugate 
  • Higher photostability compared to fluorescein, Cy5TM, Cy7TM or Alexa FluorTM 700
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides