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Product Name : Seta-633-NHS

Product Number:
K8-1663
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
633
ε [M–1 cm–1]:
250000
λEm max. [nm]:
644
QY [%]:
7
FLT [ns]:
0.25
Medium 2:
IgG D/P=1
λAb max. [nm]:
637
ε [M–1 cm–1]:
250000
λEm max. [nm]:
647
QY [%]:
26
FLT [ns]:
1.49
Medium 3:
BSA D/P=1
λAb max. [nm]:
646
λEm max. [nm]:
656
QY [%]:
51
Specs Sheet:
Download

Description

  • Highly hydrophilic, amine-reactive label containing one NHS-ester group. Brighter (ε = 250,000 M–1cm–1, QY = 26% (IgG, D/P =1)) and more photostable replacement for Alexa 633.

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
  • Fluorescence intensity and fluorescence polarization-based applications
  • Resonance Energy Transfer (RET)
  • Flow Cytometry
  • Immunofluorescence
  • Gene Expression
  • Homogeneous Assays
  • Microarrays

 

Advantages

  • Perfectly suited for excitation with the 594, 633 or 635 nm diode lasers
  • Sensitive; high extinction coefficients and quantum yields highly increase after covalent attachment to biomolecules
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility; this label does not alter the solubility of the bioconjugate 
  • High photostability; e.g. compared to fluorescein, Cy5TM or Alexa FluorTM 647
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides and amino-modified lipids