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Product Name : Seta-700-NHS

Product Number:
K8-1384
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
687
ε [M–1 cm–1]:
177000
λEm max. [nm]:
703
QY [%]:
6
Medium 2:
IgG D/P=1
λAb max. [nm]:
705
ε [M–1 cm–1]:
177000
λEm max. [nm]:
715
QY [%]:
4
Medium 3:
MeOH
λAb max. [nm]:
701
ε [M–1 cm–1]:
175000
λEm max. [nm]:
719
QY [%]:
18
Specs Sheet:
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Description

  • Water soluble, amine-reactive fluorescent label containing one reactive NHS-ester group.

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
  • Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule
  • Resonance Energy Transfer (RET)
  • Flow Cytometry
  • Immunofluorescence
  • Gene Expression
  • Homogeneous Assays
  • Assessment of protein structure

 

Advantages

  • Perfectly suited for excitation with the 380, 405, 650, 680, and 700-nm diode lasers, and UV light
  • pH-insensitive between pH 4 and pH 9
  • Good aqueous solubility; this label does not alter the solubility of the protein conjugate 
  • High photostability; e.g. compared to fluorescein or Cy5TM and Cy7TM
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified probes and amino-modified oligonucleotides