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Product Number: K8-1672

Product Name:
Seta-646-NHS
Target Group:
NH2
Excitation Light Sources:
635
Medium 1:
PB 7.4
λAb max. [nm]:
646
ε [M–1 cm–1]:
207000
λEm max. [nm]:
656
QY [%]:
10
FLT [ns]:
0.38
Medium 2:
IgG, D/P=1
λAb max. [nm]:
650
ε [M–1 cm–1]:
207000
λEm max. [nm]:
661
QY [%]:
33
Specs Sheet:
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Description

  • Highly hydrophilic, amine-reactive label containing one NHS-ester group.

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
  • Fluorescence intensity and fluorescence polarization-based applications
  • Resonance Energy Transfer (RET)
  • Flow Cytometry
  • Immunofluorescence
  • Gene Expression
  • Homogeneous Assays
  • Microarrays

 

Advantages

  • Perfectly suited for excitation with the 635 nm diode laser
  • Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules
  • Quantum yield is highly increased after covalent and non-covalent association with  proteins
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility; this label does not alter the solubility of the bioconjugate 
  • High photostability; e.g. compared to fluorescein or Cy5TM
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides and amino-modified lipids