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Product Name : Seta-650-Maleimide

Product Number:
K8-5036
Target Group:
SH
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
652
ε [M–1 cm–1]:
200000
λEm max. [nm]:
672
QY [%]:
28
Specs Sheet:
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Description

  • Highly hydrophilic, thiol-reactive label containing one maleimide group.

 

Applications

  • Covalent labeling of proteins, peptides, thiol-modified DNA and thiol-modified oligonucleotides
  • Fluorescence intensity and fluorescence polarization-based applications
  • Resonance Energy Transfer (RET)
  • Flow Cytometry
  • Immunofluorescence
  • Gene Expression
  • Homogeneous Assays
  • Microarrays

 

Advantages

  • Perfectly suited for excitation with the 635-nm and 650-nm diode lasers
  • Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility; this label does not alter the solubility of the bioconjugate 
  • High photostability compared to fluorescein, Atto 647, DyLight 650, Alexa 647 or Cy5TM
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for labeling of proteins, and thiol-modified oligonucleotides, lipids and peptides