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Product Number: K8-1667

Product Name:
Seta-635-pH-di-NHS
Target Group:
NH2
Excitation Light Sources:
635
Medium 1:
PB 7.4
λAb max. [nm]:
640
ε [M–1 cm–1]:
188000
λEm max. [nm]:
656
QY [%]:
33
FLT [ns]:
1.8
Medium 2:
IgG, D/P=1
λAb max. [nm]:
646
λEm max. [nm]:
663
QY [%]:
21
Specs Sheet:
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Description

  • Amine-reactive, pH-sensitive fluorescent label containing two reactive NHS-ester groups and pKa = 10.2 (free dye).

 

Applications

  • Covalent labeling of proteins, amino-modified DNA, amino-modified oligonucleotides and other small molecules.
  • Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule.

 

Advantages

  • Perfectly suited for excitation with the 635-nm diode laser
  • Sensitive; high extinction coefficients and high quantum yields up to 30% after covalent attachment to proteins 
  • Low non-specific binding 
  • Good aqueous solubility; this label does not alter the solubility of the protein conjugate 
  • High photostability
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified DNA probes, amino-modified oligonucleotides and other small molecules