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Product Name : Seta-750-NHS

Product Number:
K8-7522
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
753
ε [M–1 cm–1]:
230000
λEm max. [nm]:
780
QY [%]:
14
Medium 2:
IgG D/P=1
λAb max. [nm]:
756
λEm max. [nm]:
783
QY [%]:
17
Specs Sheet:
Download

Description

  • Seta-750-NHS (K8-7522) is a hydrophilic, amine-reactive label containing one NHS-ester group with almost identical absorption and emission as Cy7 or Alexa 750 and can therefore be used with these filter sets. It combines high photostability and brightness. Its extinction coefficient is 230,000 M–1cm–1.

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
  • Fluorescence intensity and fluorescence polarization-based applications
  • Resonance Energy Transfer (RET)
  • Flow Cytometry
  • Immunofluorescence
  • Gene Expression
  • Homogeneous Assays
  • Microarrays

 

Advantages

  • Perfectly suited for excitation with the 680, 700 or 750 nm diode lasers
  • Sensitive; high extinction coefficients and high quantum yields
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility; this label does not alter the solubility of bioconjugates 
  • High photostability; e.g. compared to fluorescein or Cy7
  • Low molecular weight  — Seta-750 does not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides and amino-modified lipids