Help & Support
Product Name: SeTau-647-NHS
Product Number:
K9-4149 Target Group:
NH2Excitation Light Sources:
Medium 1:
PB 7.4λAb max. [nm]:
649ε [M–1 cm–1]:
200000λEm max. [nm]:
695QY [%]:
61FLT [ns]:
3.2Medium 2:
IgG, D/P=1λAb max. [nm]:
648ε [M–1 cm–1]:
200000λEm max. [nm]:
694QY [%]:
59FLT [ns]:
3.3Specs Sheet:
Download
Description
- Extremely bright, water-soluble, amine-reactive label containing one NHS-ester group. Brightest fluorescent label currently available for the Kr-ion laser line (647 nm)
Advantages
- Perfectly suited for excitation with 635, 640, and 650-nm diode lasers
- Brightest dye currently available on the market for the red laser lines
- Low quenching tendency at high dye-to-protein/DNA ratios compared to other labels e.g. Cy5™
- Large Stokes’shift of ~46 nm (about twice that of Cy5 or Alexa 647).
- Considerably higher photostability compared to fluorescein or other cyanine dyes (Cy5 or Alexa dyes)
- High chemical stability against oxidation with peroxides or other oxygen species (e.g. ozone)
- Several times longer fluorescence lifetime (τ = 3 ns) compared to Cy5 or Alexa 647 (τ = 1 ns)
- Extremely bright label: most sensitive organic fluorescent label for proteins, amino-modified oligos or DNA currently on the market for the 647-nm Kr-ion laser line
Absorption and emission spectrum of a SeTau-647 — IgG conjugate in PBS (pH 7.4, Dye-to-protein ratio 1.0)
Total brightness (QY × ε × D/P) of SeTau-647 — IgG conjugates vs. dye-to-protein ratio (D/P) in PBS (pH 7.4) as compared to Alexa 647 — IgG and Cy5 — IgG conjugates
