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Product Number: SeTau 647 NHS

Product Name:
SeTau-647-NHS
Target Group:
NH2
Excitation Light Sources:
635 650
Medium 1:
PB 7.4
λAb max. [nm]:
649
ε [M–1 cm–1]:
200000
λEm max. [nm]:
695
QY [%]:
61
FLT [ns]:
3.2
Medium 2:
IgG, D/P=1
λAb max. [nm]:
648
ε [M–1 cm–1]:
200000
λEm max. [nm]:
694
QY [%]:
59
FLT [ns]:
3.3
Specs Sheet:
Download

Description

  • Extremely bright, water-soluble, amine-reactive label containing one NHS-ester group. Brightest fluorescent label currently available for the Kr-ion laser line (647 nm).

 

Advantages

  • Perfectly suited for excitation with 635, 640, and 650-nm diode lasers
  • Low quenching tendency at high dye-to-protein ratios compared to other labels e.g. Cy5™
  • Large Stokes’shift of ~46 nm (about twice that of Cy5 or Alexa 647).
  • Considerably higher photostability compared to fluorescein or other cyanine dyes (Cy5 or Alexa dyes)
  • High chemical stability against oxidation with peroxides or other oxygen species
  • Several times longer fluorescence lifetime (τ = 3 ns) compared to Cy5 or Alexa 647 (τ = 1 ns)
  • Extremely bright label: most sensitive organic fluorescent label for proteins currently on the market for the 647-nm Kr-ion laser line