Viscosity Sensitive Dyes

SETA BioMedicals proprietary fluorescent viscosity probes for biomedical imaging and sensing applications combine good solubility in water and aqueous buffers, high photostability, a large Stokes' shift and absorption and emission wavelengths in the visible range.

 

Excitation Light Sources Characteristics Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Medium λ abs
[nm]
ε
[M–1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                               
K8-3002
K8-3002 new
Seta-470-NHS
Seta-470-NHS
NH2 water 469 50,000 521 1.9 BSA, D/P=1 469 50,000 515 28
K8-3010
K8-3010 new
Square-460
Square-460
PB 7.4 467 50,000 515 0.9 Glycerol 470 50,100 515 43
K8-3003
K8-3003 new
Seta-470-Maleimide
Seta-470-Maleimide
SH Water 469 50,000 521 1.9 BSA, D/P=1 469 50,000 515 28

Seta-470-NHS (K8-3002) has been used to distinguish the rheological states of hydrogels and carriers in biological tissues [31].

Fluorescence area (localization) of alginate stained with Seta-470,
Seta-560 and Seta-650 and the normalized hydrogel mass versus
concentration of calcium gluconate [31]. 

 

SETA BioMedicals fluorescent molecular rotors exhibit high sensitivity as well as a large dynamic range in both the lifetime and intensity modes. They are available in reactive (NHS-ester and maleimide) and non-reactive formats.

 

Intensity-Viscosity (323x238)
Fluorescence intensity of Seta-470  
vs. viscosity
in glycerol-water = 4:1
Lifetime-Visocity(323x238).jpg;
Fluorescence lifetime of Seta-470  
vs. viscosity in glycerol-water = 4:1
 

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