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Product Name : Seta-400-NHS

Product Number:
K4-215
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
399
ε [M–1 cm–1]:
24000
λEm max. [nm]:
576
QY [%]:
34
Medium 2:
IgG D/P=1
λAb max. [nm]:
401
λEm max. [nm]:
569
QY [%]:
19
Specs Sheet:
Download

Description

  • Positively charged, water-soluble dye with one NHS-ester group and an extremely large Stokes shift

 

Applications

  • Covalent labeling of proteins, DNA and oligonucleotides
  • Resonance Energy Transfer (RET)
  • Flow Cytometry
  • Immunofluorescence
  • Homogeneous Assays

 

Advantages

  • Perfectly suited for excitation with 370-nm, 380-nm and 405-nm diode lasers
  • Extremely large Stokes' shift of over 170 nm 
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates
  • Soluble in aqueous buffers
  • Ideal for non-radioactive labeling of proteins, DNA probes and oligonucleotides