Welcome to SETA BioMedicals


SETA BioMedicals produces, commercializes and licenses proprietary fluorescent detection reagents (fluorescent dyes, probes, labels, tandems, and standards) for biomedical applications.


SETA BioMedicals also sells reactive Cy dyes  (Cy3Cy5Cy5.5 and Cy7) at highly competitive rates. Please contact us for price quotations on other derivatives or bulk orders.


These detection reagents can be ordered over the phone (217) 417 2160, via e-mail at or with our online shopping cart and are delivered the next day in the continental USA and within days at any other destination around the world.

  • Phycobiliprotein Tandem Conjugates

    Bright and photostable tandem-conjugates of APC, PE and PerCP with Seta dyes for flow cytometry and immuno-fluorescence applications.


    Flow cytometry measurment of blood cells
    stained with CD45Seta-APC-780

  • Highly Bright, Water-Soluble Rotaxane Labels

    Novel, extremely bright and photostable probes and labels based on squaraine rotaxanes. These unique fluorescent reporters combine all desirable features of common fluorophores including high chemical and photochemical stability.

    Photostability comparison of several red emitting dyes

  • Labeling Technologies

    Next generation labels for proteins, oligonucleotides and other biomolecules with exceptional brightness and photostability.


    Mouse egg cell stained with Square-655 and Square-460 Mouse egg cell stained with Square-670-Carboxy and C6-4
    Mouse egg cell stained with
    Square-655 and Square-460
    Mouse egg cell stained with

  • Click Chemistry Reagents

    Azides and proprietary DBCO derivatives of long-lifetime and 2P-imaging dyes with 2PACS of several thousand GM for Cu-catalyzed and Cu-free click chemistry reactions

  • Fluorescent Dyes for 2-Photon Microscopy

    Fluorescent probes and labels with exceptional 2-photon absorption cross-sections up to several thousand GE based on squaraines and squaraine rotaxanes

  • Luminescence Lifetime Labels

    Luminescence lifetime labels with fluorescent lifetimes from 0.5 to 32 ns for lifetime and polarization applications

  • Viscosity-Sensitive Probes and Labels

    Large Stokes' shift fluorescent molecular rotors with high sensitivity and a large dynamic range in lifetime and intensity mode

  • Fluorescence Standards

    Fluorescence standards are utilized in the calibration and validation of fluorescence instruments, luminescence readers, and as fluorescence reference materials. The standard kits offered by SETA are ready and easy to use!

  • Fluorescence Imaging

    Squaraine rotaxane dye SeTau-647 outperforms Alexa dyes (Alexa 488, Alexa 568)  in signal-to-background ratio and in vivo imaging properties.

    Quantum yields of IgG-conjugates of  SeTau-647, Alexa 647 and Cy5 at various dye-to-protein ratios in phosphate buffer (pH 7.4)
    Signal-to-background for SeTau-647 and Alexa 488 in imaging applications

  • 2-Photon Microscopy

    Compared to conventional cyanine dyes squaraine and squaraine-rotaxanes exhibit 10 – 50-times higher 2-photon cross-sections in the order of several thousand GM.


    Z-projection of a neuron filled with a squaraine rotaxane-labeled peptide
    Z-projection of a neuron filled with a squaraine rotaxane-labeled peptide

  • Superresolution Microscopy

    Seta and SeTau dyes are a class of extremely bright and phtostable dyes for fluorescence imaging and are also well suited for STED and STORM applications.


     STED image of HeLa-M cell nuclei labeled with primary antibody mAB414 and a secondary antibody labeled with SeTau-647

     STED image of HeLa-M cell nuclei labeled with primary mAB414 antibody  and a secondary antibody labeled with SeTau-647

  • DNA and Protein Microarrays

    Our highly fluorescent squaraine rotaxanes exhibit high chemical stability in particular against oxidative reagents such as peroxides or ozone and therefore are excellent detection reagents for DNA and protein microarrays where the fluorescence signals of cyanine dyes such as Cy5 and  Alexa 647 are strongly dependent on the concentration of ozone during post-hybridization array washing.

  • Free Consultation

    SETA BioMedicals offers free consultation on the use of SETA products for your application. Please call us at 217 417 2160 or use LiveChat.