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Plasmonics

 Seta and SeTau dyes exhibit larger sensitivity enhancements than any other type of dyes on plasmonic platforms.

 

Excitation Light Sources Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                   
K8-1342
K8-1342
Seta-670-NHS
Seta-670-NHS
NH2 PB 7.4 667 180000 688 7 0.42
K8-1642
K8-1642
Seta-632-NHS
Seta-632-NHS
NH2 PB 7.4 632 280000 641 6
K8-1663
K8-1663
Seta-633-NHS
Seta-633-NHS
NH2 PB 7.4 633 250000 644 7 0.25
K9-4119
K9-4119 new
SeTau-665-NHS
SeTau-665-NHS
NH2 PB 7.4 664 160000 712 53 3.1
K9-4142
K9-4142
SeTau-647-di-NHS
SeTau-647-di-NHS
NH2 PB 7.4 650 200000 694 65 3.2
K9-4149
K9-4149
SeTau-647-NHS
SeTau-647-NHS
NH2 PB 7.4 649 200000 695 61 3.2
K8-1341
K8-1341
Seta-670-Maleimide
Seta-670-Maleimide
SH PB 7.4 667 180000 688 7

 

Metal Enhanced Fluorescence (MEF)

Metal-enhanced fluorescence (MEF) is an important approach to increase the sensitivity thereby decreasing the detection limit of biomedical assays. There are two contributions to MEF. Firstly, under the influence of the incident light, the molecule goes to the excited state and interacts with the metal. Secondly, metal particles accelerate the fluorophore’s radiative decay rate and as a result enhance the emission. This emitter-antenna-like effect results in an increase of the quantum yield of the molecule and in a decrease of its lifetime. A simultaneous increase of the quantum yield and decrease of the lifetime is only possible when the radiative rate increases. Therefore this effect is also known as radiative decay engineering (RDE). While typical enhancement levels for conventional dyes on plasmonic platforms are in the order of 5-7-fold, SeTau-665 shows a 70-fold enhancement in ensemble measurements (Figure) and up to 400-fold in certain ‘hot spots’ in single molecule measurements [16]. Such enhancement levels have not been observed with any other type of dyes.

 

Emission spectra of SeTau-665 labeled antibody on a quartz (Glass) and self-assembled colloidal silver (SACS) surface.
Emission spectra of SeTau-665 labeled antibody on a quartz (Glass) and self-assembled colloidal silver (SACS) surface. Upper panels exhibit real-color photograph of labeled IqG spotted on the silver (left) and quartz (right) surfaces

Due to the large enhancements in single molecule (SM) level measurements, SM studies which were previously impossible can be successfully performed on such plasmonic platforms using SeTau dyes.

The large increase in brightness allows the reduction of the excitation power resulting in a reduced background and increased photostability. The remarkable fluorescence enhancements observed for SeTau dyes on plasmonic platform substantially improves single molecule detection and reduces the detection limits in sensing devices. 

 

Product Number
(Specs Sheet)
Product Name
(Product Info)
K9-4142 SeTau-647-di-NHS
K9-4149 SeTau-647-NHS

 


Seta and SeTau dyes show much higher signal enhancement (SE) on silver surfaces (SIFs) compared to fluorophores such as Cy5, Alexa 647 or phycobiliproteins (PE, APC):
 
SIF-coated vs. non-coated glass fluorescence signal ratio at various wavelengths tested in a model immunoassay for SeTau dyes (SE ~ 70 times in bulk and SE ~ 400 times in single molecule measurements), Seta-670-NHS, Seta-633-NHS or Seta-632-NHS (SE ~ 15 – 20 times) vs. Cy5, Alexa 647 or APC (SE ~ 6 - 7 times).

SIF-coated vs. non-coated glass fluorescence signal ratio at various wavelengths tested in a model immunoassay for SeTau dyes (SE ~ 70 times in bulk and SE ~ 400 times in single molecule measurements), Seta-670-NHS, Seta-633-NHS or Seta-632-NHS (SE ~ 15 – 20 times) vs. Cy5, Alexa 647 or APC (SE ~ 6 - 7 times).
 

 

Product Number
(Specs Sheet)
Product Name
(Product Info)
K8-1342 Seta-670-NHS
K8-1663 Seta-633-NHS
K8-1642 Seta-632-NHS

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