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Product Number: K8-1696

Product Name:
Seta-633-Azide
Target Group:
triple-CC
Excitation Light Sources:
594 635
Medium 1:
PB 7.4
λAb max. [nm]:
633
ε [M–1 cm–1]:
250000
λEm max. [nm]:
644
QY [%]:
7
Medium 2:
PB 7.4
λAb max. [nm]:
633
ε [M–1 cm–1]:
250000
λEm max. [nm]:
644
QY [%]:
7
Specs Sheet:
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Description

  • Highly hydrophilic, alkyne-reactive, long-lifetime fluorescent reagent for click chemistry containing one azide function. Azides react with C≡C-triple bonds in either a Cu(I)-catalyzed or Cu-free 1,3-dipolar cycloaddition reaction to triazole.

 

Applications

  • Click Chemistry reagent
  • Fluorescence intensity and fluorescence polarization-based applications
  • Resonance Energy Transfer (RET)

 

Advantages

  • Perfectly suited for excitation with the 633 or 635-nm diode lasers
  • Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules
  • Quantum yield is highly increased after covalent and non-covalent association with proteins
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility: this label does not alter the solubility of the bioconjugate 
  • Photostability: Higher photostability as compared to Alexa Fluor™ 647 or Cy5
  • Low molecular weight: Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of alkyne-modified proteins, DNA and oligonucleotides