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Product Name : Seta-670-Maleimide

Product Number:
K8-1341
Target Group:
SH
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
667
ε [M–1 cm–1]:
180000
λEm max. [nm]:
688
QY [%]:
7
Specs Sheet:
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Description

  • Highly hydrophilic, thiol-reactive fluorescent label containing one reactive maleimide group.

 

Applications

  • Covalent labeling of proteins, thiol-modified DNA and thiol-modified oligonucleotides
  • Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule
  • Fluorescence Resonance Energy Transfer (FRET) applications
  • Single Molecule Applications — Seta-670 shows extreme low blinking in single molecule measurements
  • Flow Cytometry
  • Immunofluorescence
  • Gene Expression
  • Homogeneous Assays
  • Assessment of protein structure

 

Advantages

  • Perfectly suited for excitation with the 380, 404, 635, 670-nm diode lasers, LEDs, and UV light
  • Sensitive; high extinction coefficients and high quantum yields up to 50% after covalent attachment to proteins
  • Quantum yield is increased when covalently and non-covalently bound to protein
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility; this label does not alter the solubility of the protein conjugate 
  • High photostability; e.g. compared to fluorescein or Cy5TM
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, thiol-modified DNA probes and thiol-modified oligonucleotides