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Product Name : SeTau-647-di-NHS

Product Number:
K9-4142
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
650
ε [M–1 cm–1]:
200000
λEm max. [nm]:
694
QY [%]:
65
FLT [ns]:
3.2
Medium 2:
IgG, D/P=0.5
λAb max. [nm]:
649
ε [M–1 cm–1]:
200000
λEm max. [nm]:
691
QY [%]:
58
FLT [ns]:
3.2
Specs Sheet:
Download

Description

  • Water-soluble, amine-reactive label containing two NHS-ester groups — brightest fluorescent label currently available for the 647-nm Kr-ion laser line

 

Applications

  • Fluorescence imaging
  • Fluorescence lifetime applications and fluorescence lifetime imaging (FLIM)
  • Superresolution microscopy
  • Immunofluorescence and FRET applications
  • 2-Photon microscopy
  • Single molecule applications
  • Fluorescence correlation spectroscopy (FCS)
  • Proteomics

 

Advantages

  • Perfectly suited for excitation with the 635-nm, 647-nm, or 650-nm lasers
  • Brightest dye currently available on the market for the red laser lines
  • Highly sensitive: high extinction coefficient of 200,000 M–1cm–1 and high quantum yield of ~60% in aqueous environments
  • Good aqueous solubility: this label does not alter the solubility of the protein conjugate
  • Ozone stability: Higher ozone stability than Alexa Fluor™ 647 or Cy5™ enables array experiments to be performed with SeTau 647 under any environmental condition
  • Large Stokes' shift: SeTau647 has a 44 nm Stokes' shift
  • Extremely photostable: Much higher photostability as compared to Alexa Fluor™ 647, DyLight 650 , ATTO 647N or Cy5™ (see below)
  • Fluorescence lifetime three times longer than that of Cy5™ or Alexa Fluor™ 647: 3.1 ns in water
  • Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides