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Albumin Determination

Excitation Light Sources Characteristics Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Medium λ abs
[nm]
ε
[M–1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                             
K35
K35
K35
K35
Toluene 410 10000 495 60 EtOH 430 13200 552 0.01
K8-1355
K8-1355
Square-680-Carboxy
Square-680-Carboxy
PB 7.4 654 138000 670 1 1g/L BSA 688 168000 708 62
K8-1500
K8-1500
Square-655
Square-655
PB 7.4 622 177000 644 0.09 1 g/L BSA 652 188,000 672 80

K35 is a fluorescent probe for determination of albumin binding sites in plasma and serum [19, 20].

Square dyes exhibit high extinction coefficients and lower quantum yields in aqueous buffer but high quantum yields and long fluorescence lifetimes in presence of serum albumin. As an example Square-655 is a fluorescent probe with an extinction coefficient of 177 000 M-1cm-1 and a quantum yield of 80% in presence of albumin. These features make them the most sensitive fluorescent probes for the measurement of not only albumin but also other proteins. Because the binding constants of Square dyes to albumin are of the same order as for some of the common drugs these dyes are also useful for the estimation of drug-binding constants using the dye-displacement method.

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