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Product Number: K8-7045

Product Name:
Seta-750-DBCO
Target Group:
N3
Excitation Light Sources:
680 700 750
Medium 1:
PB 7.4
λAb max. [nm]:
754
ε [M–1 cm–1]:
234000
λEm max. [nm]:
782
QY [%]:
21
Specs Sheet:
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Description

  • Seta 750-DBCO (K8-7045) is a highly hydrophilic click chemistry reagent containing one dibenzocyclo-octyne (DBCO) group for strain-mediated cycloaddition reactions with azides. It features the same excitation and emission wavelengths as Cy7, Alexa Fluor™ 750 or Seta 750 and can therefore be used with these filter sets. It combines high photostability and brightness.

 

Applications

  • Strain-mediated click chemistry reactions with azide-modified reagents and biomolecules.

 

Advantages

  • Perfectly suited for excitation with the 680, 700 or 750 nm diode lasers
  • Sensitive; high extinction coefficients and high quantum yields (about twice as high as Alexa 750)
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility 
  • High photostability; e.g. compared to fluorescein, Cy7 or Alexa 750
  • Low molecular weight  — Seta-750 does not add substantial mass to the conjugates