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Genomics

Genomics  is a branch of molecular biology relating to the structure, function, evolution, and mapping of genomes and more specifically relates to DNA, DNA sequencing methods and bioinformatics.

Below is a list of fluorescent labels (specifically maleimides) including Zy-dyes  that are commonly used for labelling of thiol-modified oligonucleotieds. They also include labels with long lifetimes for fluorescence lifetimes (FLT) and/or fluorescence polarization (FP) applications.

Excitation Light Sources Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                   
K8-1641
K8-1641
Seta-632-Maleimide
Seta-632-Maleimide
SH PB 7.4 633 270000 642 5
K8-5036
K8-5036
Seta-650-Maleimide
Seta-650-Maleimide
SH PB 7.4 652 200000 672 28
K9-4148
K9-4148
SeTau-647-Maleimide
SeTau-647-Maleimide
SH PB 7.4 648 200000 692 45 3.2
K8-3403
K8-3403
Zy3-Maleimide
Zy3-Maleimide
SH PB 7.4 549 150000 564 4 0.3
K8-5403
K8-5403
Zy5-Maleimide
Zy5-Maleimide
SH PB 7.4 647 250000 665 27 1
K8-5423
K8-5423
Zy5.5-Maleimide
Zy5.5-Maleimide
SH PB 7.4 676 190000 694 23 1
K8-1341
K8-1341
Seta-670-Maleimide
Seta-670-Maleimide
SH PB 7.4 667 180000 688 7
K8-1671
K8-1671
Seta-646-Maleimide
Seta-646-Maleimide
SH PB 7.4 647 210000 657 8
K7-548
K7-548
SeTau-405-Maleimide
SeTau-405-Maleimide
SH PB 7.4 405 13800 518 51 9.1
K8-2100
K8-2100 new
YOSeta-1 (1mM solution in DMSO)
YOSeta-1 (1mM solution in DMSO)
dsDNA 492 98800 509 52
K8-3003
K8-3003 new
Seta-470-Maleimide
Seta-470-Maleimide
SH Water 469 50000 521 1.9

new Staining of double-stranded DNA: YOSeta-1 is a cell-impermeant fluorescent dye used for staining of double-stranded DNA similar to YOYO-1. Unlike YOYO-1, YOSeta-1 is highly soluble in aqueous buffers. The free YOSeta-1 dye has a very low fluorescence quantum yield but the QY increases several thousand times upon binding to double-stranded DNA (see specs sheet). The probe is available as a 1 mM solution in DMSO.

YOSeta-1-dsDNA

Absorption and emission spectrum of YOSeta-1 in presence of dsDNA

 

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