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Product Number: K8-7046

Product Name:
Seta-750-Azide
Target Group:
triple-CC
Excitation Light Sources:
680 700 750
Medium 1:
PB 7.4
λAb max. [nm]:
750
ε [M–1 cm–1]:
200000
λEm max. [nm]:
779
QY [%]:
22
Specs Sheet:
Download

Description

  • Hydrophilic, alkyne-reactive, long-lifetime reagent for click chemistry containing one azide function. Azides react with C≡C-triple bonds in either a Cu(I)-catalyzed or Cu-free 1,3-dipolar cycloaddition reaction to triazole.

 

Applications

  • Click Chemistry reagent
  • Fluorescence intensity and fluorescence polarization-based applications
  • Resonance Energy Transfer (RET)

 

Advantages

  • Perfectly suited for excitation with the 680, 700 or 750 nm diode lasers
  • Sensitive; high extinction coefficients and high quantum yields
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility: this label does not alter the solubility of the bioconjugate 
  • Photostability: Higher photostability as compared to Cy7TM
  • Low molecular weight: Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of alkyne-modified proteins, DNA and oligonucleotides