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Product Name : Seta-405 NHS

Product Number:
K6-5052
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
401
ε [M–1 cm–1]:
30000
λEm max. [nm]:
452
QY [%]:
70
Medium 2:
IgG D/P=1
λAb max. [nm]:
405
λEm max. [nm]:
453
QY [%]:
56
Specs Sheet:
Download
  • Hydrophilic, amine-reactive label containing one NHS-ester group

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
  • Resonance Energy Transfer (RET)
  • Flow Cytometry
  • Immunofluorescence
  • Gene Expression
  • Homogeneous Assays
  • Assessment of protein structure

 

Advantages

  • Perfectly suited for excitation with the 380 and 405-nm diode lasers and UV light
  • High quantum yields of up to 70%
  • Large Stokes' shift
  • High photostability; e.g. compared to fluorescein
  • Low molecular weight — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides