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Product Name : Seta-632-Maleimide

Product Number:
K8-1641
Target Group:
SH
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
633
ε [M–1 cm–1]:
270000
λEm max. [nm]:
642
QY [%]:
5
Specs Sheet:
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Description

  • Highly hydrophilic, thiol-reactive fluorescent label containing one reactive maleimide group

 

Applications

  • Covalent labeling of thiol-modified peptides, oligonucleotides, and proteins
  • Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule
  • Fluorescence intensity and fluorescence polarization-based applications

 

Advantages

  • Perfectly suited for excitation with the 635 nm diode lasers
  • Sensitive; high extinction coefficients andhigh quantum yields after covalent attachment to biomolecules
  • Quantum yield is highly increased after covalent and non-covalent association with  proteins
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility; this label does not alter the solubility of the bioconjugate
  • High photostability
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of thiol-modified proteins, peptides, and oligonucleotides