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Seta Labels

Seta dyes are in general more hydrophilic than Square dyes as they are less environment-sensitive. The Seta dyes series spans the whole visible range and the NIR.

Excitation Light Sources Characteristics Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Medium λ abs
[nm]
ε
[M–1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                               
K8-1342
K8-1342
Seta-670-NHS
Seta-670-NHS
NH2 PB 7.4 667 180000 688 7 0.42 BSA, D/P=1.5 681 180000 696 27 2.43
K8-1346
K8-1346
Seta-670-Azide
Seta-670-Azide
triple-CC PB 7.4 667 180000 686 7 PB 7.4 667 180000 686 7
K8-1367
K8-1367
Seta-670-pH-di-NHS
Seta-670-pH-di-NHS
NH2 PB 6.0 672 93000 694 19 IgG, D/P=1 684 703 7
K8-1384
K8-1384
Seta-700-NHS
Seta-700-NHS
NH2 PB 7.4 687 177000 703 6 IgG D/P=1 705 177000 715 4
K8-1626
K8-1626
Seta-633-di-NHS
Seta-633-di-NHS
NH2 PB 7.4 635 192000 644 12 0.7 IgG, D/P=1 639 192000 650 33 3
K8-1641
K8-1641
Seta-632-Maleimide
Seta-632-Maleimide
SH PB 7.4 633 270000 642 5
K8-1642
K8-1642
Seta-632-NHS
Seta-632-NHS
NH2 PB 7.4 632 280000 641 6 IgG, D/P=1 637 647 18
K8-1663
K8-1663
Seta-633-NHS
Seta-633-NHS
NH2 PB 7.4 633 250000 644 7 0.25 IgG D/P=1 637 250000 647 26 1.49
K8-1667
K8-1667
Seta-635-pH-di-NHS
Seta-635-pH-di-NHS
NH2 PB 7.4 640 188000 656 33 1.8 IgG, D/P=1 646 663 21
K8-1672
K8-1672
Seta-646-NHS
Seta-646-NHS
NH2 PB 7.4 646 207000 656 10 0.38 IgG, D/P=1 650 207000 661 33
K8-1682
K8-1682
Seta-660-di-NHS
Seta-660-di-NHS
NH2 PB 7.4 661 220000 672 11 0.9 IgG D/P=1 665 220000 675 49
K8-1696
K8-1696
Seta-633-Azide
Seta-633-Azide
triple-CC PB 7.4 633 250000 644 7 PB 7.4 633 250000 644 7
K8-3002
K8-3002 new
Seta-470-NHS
Seta-470-NHS
NH2 water 469 50000 521 1.9 BSA, D/P=1 469 515 28
K8-3335
K8-3335
Seta-555-NHS
Seta-555-NHS
NH2 PB 7.4 555 155000 570 7 IgG, D/P=1 556 571 13
K8-3345
K8-3345
Seta-555-DBCO
Seta-555-DBCO
N3 PB 7.4 555 155000 570 7
K8-3346
K8-3346
Seta-555-Azide
Seta-555-Azide
triple-CC PB 7.4 555 155000 570 7
K8-5035
K8-5035
Seta-650-NHS
Seta-650-NHS
NH2 PB 7.4 651 200000 671 28 IgG, D/P=1 654 673 37
K8-5036
K8-5036
Seta-650-Maleimide
Seta-650-Maleimide
SH PB 7.4 652 200000 672 28
K8-5045
K8-5045
Seta-650-DBCO
Seta-650-DBCO
N3 PB 7.4 653 200000 674 28
K8-5046
K8-5046
Seta-650-Azide
Seta-650-Azide
triple-CC PB 7.4 651 200000 671 28
K8-5075
K8-5075
Seta-680-NHS
Seta-680-NHS
NH2 PB 7.4 678 200000 698 24 IgG, D/P=1 680 701 30
K8-7045
K8-7045
Seta-750-DBCO
Seta-750-DBCO
N3 PB 7.4 754 234000 782 21
K8-7046
K8-7046
Seta-750-Azide
Seta-750-Azide
triple-CC PB 7.4 750 200000 779 22
K8-7055
K8-7055
Seta-780-NHS
Seta-780-NHS
NH2 PB 7.4 777 215000 807 1.2
K8-1388
K8-1388
Seta-700-di-NHS
Seta-700-di-NHS
NH2 PB 7.4 688 180000 704 11 IgG D/P=1 696 713 23
K8-1341
K8-1341
Seta-670-Maleimide
Seta-670-Maleimide
SH PB 7.4 667 180000 688 7
K8-1671
K8-1671
Seta-646-Maleimide
Seta-646-Maleimide
SH PB 7.4 647 210000 657 8 IgG, D/P=1 650 661 31
K4-205
K4-205 new
Seta-375-NHS
Seta-375-NHS
NH2 PB 7.4 372 24500 476 86 3.1 IgG, D/P=5 372 24500 480 42 3.5
K8-7522
K8-7522
Seta-750-NHS
Seta-750-NHS
NH2 PB 7.4 753 230000 780 14 IgG D/P=1 756 783 17
K8-3003
K8-3003 new
Seta-470-Maleimide
Seta-470-Maleimide
SH Water 469 50000 521 1.9 EtOH 462 50000 515 2.2
K4-225
K4-225 new
Seta-385-NHS
Seta-385-NHS
NH2 PB 7.4 384 25000 512 78 IgG, D/P=6 387 25000 505 29
K8-1389
K8-1389 new
Seta-700-NHS
Seta-700-NHS
NH2 PB 7.4 688 162000 703 14 IgG D/P=1 694 710 18
K6-5052
K6-5052 new
Seta-405 NHS
Seta-405 NHS
NH2 PB 7.4 401 30000 452 IgG D/P=1 405 553 56
K4-215
K4-215 new
Seta-400-NHS
Seta-400-NHS
NH2 PB 7.4 399 24000 576 34 IgG D/P=1 401 569 19

Seta-375-NHS is an extremely bright, reactive label for use with the UV 375 - 380 nm diode lasers. It is much brighter alternative for Alexa 350 with a much higher extiction coefficient and quantum efficiency. Exc./Em. max: 372/480 nm.

Seta-470-NHS is a fluorescent label perfectly suited for the 447, 457 and 473 nm diode lasers. Its extinction coefficient is 50,000 M–1cm–1 and it exhibits highly increased QYs after covalent attachment to proteins. Exc./Em. max: 469/515 nm.

Seta-555-NHS is a direct replacement for Cy3, Alexa 555 or DyLight 550 and can therefore be used with these filter sets. Its extinction coefficient is 154,000 M–1cm–1 and the QY of Seta-555-NHS is about twice as high as that of Cy3.

Seta-632-NHS is a better alternative to Alexa 633: it has an extinction coefficient of 280,000 M–1cm–1 as compared to 159,000 M–1cm–1 for Alexa 633. Emitting bright red fluorescence this dye can be measured with the Alexa 633 filter set. Exc./Em. max of IgG-conjugates: 637/647 nm.

Seta-633-NHS is a hydrophilic, mono-reactive label for proteins and other biomolecules. It is extremely bright and has a low tendency for self-quenching even at higher D/P ratios. The extinction coefficient is 250,000 M–1cm–1as compared to 159,000 M–1cm–1 for Alexa 633 or 170,000 M–1cm–1 for DyLight 633, and quantum yields as high as 70% were measured with conjugates of this long-wavelength dye. It is optimally suited to be excited with the 633 nm spectral line of the He-Ne laser or the 635 nm line of red diode lasers and replaces Alexa 633 and/or DyLight 633. Exc./Em. max of IgG-conjugates: 637/647 nm.

Seta-646-NHS is extremely bright (extinction coefficient is ~210,000 M–1cm–1, and the quantum yield of labeled antibodies are as high as 33% at a D/P ~ 1) and an ideal replacement of Cy5, Alexa 647, or DyLight 650 producing intense red fluorescence emission. It is also extremely photostable and can be optimally excited with the 647 nm spectral line of the Krypton-Argon laser. Exc./Em. max for conjugates: 650/661 nm.

Seta-650-NHS is a brighter (extinction coefficient is ~200,000 M–1cm–1, and the quantum yield of labeled antibodies are as high as 33% at a D/P ~ 1) replacement for Cy5 or DyLight 650 producing intense red fluorescence emission. It is a highly photostable dye that is optimally excited with the 647 nm spectral line of the Krypton-Argon laser. Exc./Em. max for conjugates: 650/671 nm.

Seta-650 (K8-5035) and Seta-750 (K8-7035)-conjugated PEG-single walled carbon nanotubes (PEG-SWCNT-650 or PEG-SWCNT-750) were produced by labeling the PEG terminal amino groups on amino-modified PEG-SWCNTs with either Seta-650-NHS or Seta-750-NHS.
Cartilage ECM trafficking and chondrocyte internalization of injected PEG-SWCNTs: Polyethylene-Glycol-Modified Single-Walled Carbon Nanotubes for Intra-Articular Delivery to Chondrocytes, M. Bottini et al. ACS Nano, 2014, 8 (12), 12280–12291.

Seta-660-di-NHS is much brighter alternative to Alexa 660: Its extinction coefficient is 220,000 M–1cm–1 as compared to 132,000 M–1cm–1 for Alexa 660, and quantum yields as high as 50% were measured from IgG-conjugates with this long-wavelength marker. It emits intense fluorescence in the far-red region of the light spectrum. Ex./Em. max: 661/672 nm.

Seta-680-NHS is a dye that replaces Cy5.5, Alexa 680 or DyLight 680. The dye has an extinction coefficient of 200,000 M–1cm–1 as compared to 160,000 M–1cm–1 for DyLight 680 and its IgG-conjugates are bright and photostable. Ex./Em. max: 678/698 nm.

Seta-700-NHS is a bright, water-soluble fluorescent label with a Q.Y. of up to 20% when labeled to IgG. Replaces Alexa 700 (QY = 0.13). Ex./Em. max (IgG-Conjugate): 694/710 nm.

Seta-750-NHS is a hydrophilic, mono-reactive label with almost identical absorption and emission as Cy7 or Alexa 750 and can therefore be used with these filter sets. It combines high photostability and brightness. Replacement for Cy7, Alexa 750 and DyLight 750. Its extinction coefficient is 230,000 M–1cm–1. Ex./Em. max: 753/780 nm.

In-vivo analysis of the biodistribution of fluorescently labelled PEG-single wall carbon nanotubes: Panels show recording of fluorescence in mice injected with PBS (a) Seta750 (b) and PEG-SWCNT-750 (c). No fluorescence was recorded in control mice (a) at any time. Already 10 min after the injection, animals that received the fluorophore Seta750 alone (b) displayed fluorescence almost exclusively localized in the bladder. High fluorescence was recorded in liver and brown fat of mice administered with PEG-SWCNT-750 (c); one hour after the injection bladder accumulation of fluorescence was also visible in this group. From: M. Bottini et al. Biodistribution and toxicity of pegylated single wall carbon nanotubes in pregnant mice. Particle and Fibre Toxicology 2013 10:21.

C. Sacchetti et al. Surface Polyethylene Glycol Conformation Influences the Protein Corona of Polyethylene Glycol-Modified Single-Walled Carbon Nanotubes: Potential Implications on Biological Performance. ACS Nano. 2013 Mar 26;7(3):1974-89.

 All dyes are available in the following formats: carboxyl, NHS-esters and maleimides.

 

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