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Product Name : Seta-650-NHS

Product Number:
K8-5035
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
651
ε [M–1 cm–1]:
200000
λEm max. [nm]:
671
QY [%]:
28
Medium 2:
IgG, D/P=1
λAb max. [nm]:
654
λEm max. [nm]:
673
QY [%]:
37
Specs Sheet:
Download

Description

  • Highly hydrophilic, amine-reactive label containing one NHS-ester group.

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
  • Fluorescence intensity and fluorescence polarization-based applications
  • Resonance Energy Transfer (RET)
  • Flow Cytometry
  • Immunofluorescence
  • Gene Expression
  • Homogeneous Assays
  • Microarrays

Advantages

  • Perfectly suited for excitation with the 635-nm and 650-nm diode lasers
  • Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules
  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility; this label does not alter the solubility of the bioconjugate 
  • High photostability compared to fluorescein, Atto 647, DyLight 650, Alexa 647 or Cy5TM
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides and amino-modified lipids