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Product Name : SeTau-680-NHS

Product Number:
K9-4179
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
683
ε [M–1 cm–1]:
215000
λEm max. [nm]:
705
QY [%]:
58
FLT [ns]:
2.9
Medium 2:
IgG D/P=1
λAb max. [nm]:
682
λEm max. [nm]:
703
QY [%]:
47
FLT [ns]:
3.1
Specs Sheet:
Download

Description

  • Extremely bright, water-soluble, amine-reactive squaraine-rotaxane label containing one NHS-ester group. The ideal label for proteins and other amino-modified biomolecules including oligonucleotides.

 

Advantages

  • Perfectly suited for excitation with 635, 650, 670 and 680-nm diode lasers
  • Low quenching tendency at high dye-to-protein ratios compared to other labels e.g. Cy5.5™
  • Considerably higher photostability compared to other cyanine dyes (Cy5.5 or Alexa 680)
  • High chemical stability against oxidation with peroxides or other oxygen species
  • Longer fluorescence lifetime (τ ~ 3 ns) compared to Cy5.5 (τ~ 1 ns) or Alexa 680 (τ~ 1.2 ns)
  • Extremely bright label: SeTau 680: (Φ = 0.58, ε = 215,000 and Φ. ε =124,700) vs Alexa 680 (Φ = 0.36, ε = 183,000 and Φ. ε = 65,880)