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Product Name : Seta-650-DBCO

Product Number:
K8-5045
Target Group:
N3
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
653
ε [M–1 cm–1]:
200000
λEm max. [nm]:
674
QY [%]:
28
Specs Sheet:
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Description

  • Highly hydrophilic, azide-reactive reagent containing one dibenzocyclo-octyne (DBCO) functionality for strain-medicated Cu-free click chemistry reactions.

 

Applications

  • Cu-free click chemistry reaction with azide-modified proteins, azide-modified DNA and azide-modified oligonucleotides
  • Fluorescence intensity and fluorescence polarization-based applications
  • Resonance Energy Transfer (RET)
  • Flow Cytometry
  • Immunofluorescence
  • Gene Expression
  • Homogeneous Assays
  • Microarrays

 

Advantages

  • Perfectly suited for excitation with 635, 647 or 650-nm diode lasers
  • Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules
  • pH-insensitive between pH 3 and pH 10
  •  Good aqueous solubility; this label does not alter the solubility of a bioconjugate
  • High photostability; e.g. compared to Cy5™
  • Ideal for non-radioactive labeling of azide-modified molecules (drugs, proteins, oligos, lipids and DNA)