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Flow Cytometry

Flow Cytometry is a powerful technique to measure multiple parameters from cell populations. Flow cytometers differentiate cells based on their size, internal complexity, and fluorescence signature. Qualitative and quantitative analyses of cell populations have relevance for clinical and research applications.

Excitation Light Sources Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                   
K7-547
K7-547
SeTau-405-NHS
SeTau-405-NHS
NH2 PB 7.4 405 13800 518 80 9.3
K7-545
K7-545
SeTau-425-NHS
SeTau-425-NHS
NH2 PB 7.4 425 4200 545 39 26.2
K8-1384
K8-1384
Seta-700-NHS
Seta-700-NHS
NH2 PB 7.4 687 177000 703 6
K8-1626
K8-1626
Seta-633-di-NHS
Seta-633-di-NHS
NH2 PB 7.4 635 192000 644 12 0.7
K8-1682
K8-1682
Seta-660-di-NHS
Seta-660-di-NHS
NH2 PB 7.4 661 220000 672 11 0.9
K8-5035
K8-5035
Seta-650-NHS
Seta-650-NHS
NH2 PB 7.4 651 200000 671 28
K8-5036
K8-5036
Seta-650-Maleimide
Seta-650-Maleimide
SH PB 7.4 652 200000 672 28
K8-7055
K8-7055
Seta-780-NHS
Seta-780-NHS
NH2 PB 7.4 777 215000 807 1.2
K9-4119
K9-4119 new
SeTau-665-NHS
SeTau-665-NHS
NH2 PB 7.4 664 160000 712 53 3.1
K9-4149
K9-4149
SeTau-647-NHS
SeTau-647-NHS
NH2 PB 7.4 649 200000 695 61 3.2
K8-1388
K8-1388
Seta-700-di-NHS
Seta-700-di-NHS
NH2 PB 7.4 688 180000 704 11
K8-1341
K8-1341
Seta-670-Maleimide
Seta-670-Maleimide
SH PB 7.4 667 180000 688 7
K4-205
K4-205 new
Seta-375-NHS
Seta-375-NHS
NH2 PB 7.4 372 24500 476 86 3.1
K7-548
K7-548
SeTau-405-Maleimide
SeTau-405-Maleimide
SH PB 7.4 405 13800 518 51 9.1
cl-APC
cl-APC
Crosslinked APC
Crosslinked APC
PB 7.4 650 700000 662
R-PE
R-PE
Lyophilized R-PE
Lyophilized R-PE
PB 7.4 565 1960000 573
K10-6801
K10-6801 new
Seta-PerCP-680
Seta-PerCP-680
PB 7.4 481 380000 676
K10-7801
K10-7801 new
Seta-APC-780
Seta-APC-780
PB 7.4 651 1000000 780
K10-6701
K10-6701 new
Seta-R-PE-670
Seta-R-PE-670
PB 7.4 488 1100000 671
K10-0775
K10-0775 new
Seta-R-PE-775
Seta-R-PE-775
PB 7.4 488 1100000 774
K10-7151
K10-7151 new
Seta-APC-715
Seta-APC-715
PB 7.4 651 600000 713
K7-544
K7-544
SeTau-425-Maleimide
SeTau-425-Maleimide
SH PB 7.4 425 4200 545 39 26.2
K4-225
K4-225 new
Seta-385-NHS
Seta-385-NHS
NH2 PB 7.4 384 25000 512 78
K6-5052
K6-5052 new
Seta-405 NHS
Seta-405 NHS
NH2 PB 7.4 401 30000 452
K4-215
K4-215 new
Seta-400-NHS
Seta-400-NHS
NH2 PB 7.4 399 24000 576 34

Seta-405 is identical with Pacific Blue with excitation and emission at 401/452 nm.

SeTau-405 is a spectral analog of Pacific Orange with excitation and emission at 405/518 nm.

SeTau-647 is an extremely bright label unsurpassed by any other red dye for excitation with the 633 nm laser. Its Q.Y. in aqueous solutions is 60% and its Stokes' shift is > 40 nm.

Seta-700 has an extremely large Stokes shift of 300 nm and is therefore used as a third color in the 405 nm channel. Its extinction at 405 nm is around 40,000 M-1cm-1

Seta-R-PE-670, Seta-PerCP-680 and Seta-R-PE-775 are our latest editions to this product line.

Seta-APC-780 is a much brighter and a much more photostable replacement for APC-Cy7.

 Peripheral blood samples stained with CD81 — Seta-APC-780
 Peripheral blood samples stained with CD45 — Seta-APC-780
 Peripheral blood samples stained with CD3 — Seta-APC-780
Plasma cells stained with CD38 — Seta-APC-780

Top and bottom left: Peripheral blood samples stained with CD45-, CD81- and
CD3 — Seta-APC-780; Bottom right: plasma cells stained with CD38 — Seta-APC-780

Below is a table listing of our proprietary replacements for the most common fluorophores/tandems currently used in flow cytometry: 

 

Laser Source Labels Used Superior Seta Replacements

405 nm

Pacific Blue Seta-405
Pacific Orange SeTau-405

488 nm

R-PE Lyophilized R-PE
R-PE - Texas Red coming soon!
R-PE - Cy5 Seta-R-PE-670
 PerCP - Cy5.5, R-PE - Cy5.5 Seta-PerCP-680
R-PE - Cy7 Seta-R-PE-775

633 nm

APC, Alexa 647  Lyophilized clAPCSeta-650
 APC - Cy5.5 SeTau-647, APC - Seta-680, APC - SeTau-647
Alexa 700 Seta-700
APC - Cy7 Seta-APC-780


For a specific listing and more detailed information about these tandems we refer you to tandem-conjugates.

Please contact us if you are interested in a sample for testing.

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