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Phycobiliproteins

Phycobiliproteins are water-soluble, light-harvesting proteins that are highly fluorescent due to the incorporation of tetrapyrrole chromophores. They are isolated from cyanobacteria and eukaryotic algae. Phycobiliproteins hold the record for the largest number of photons emitted before bleaching – they are one of the most sensitive fluorescent reporters known. They combine extremely high extinction coefficients and quantum yields close to unity (see below). Because of their high sensitivity, phycobiliprotein-labeled antibodies and other molecules are used in fluorescence applications such as flow cytometry and immuno-blotting/immuno-staining. They are also used as energy transfer donors in tandem-constructs for multi-color analysis in flow cytometry.

Main classes of phycobiliproteins: Allophycocyanin, R-Phycocyanin, B-Phycoerythrin, R-Phycoerythrin:
 

 Red Algae  Cyanobacteria

Main classes of phycobiliproteins: Allophycocyanin, R-Phycocyanin, B-Phycoerythrin, R-Phycoerythrin

SETA BioMedicals offers lyophilized phycobiliproteins.

Excitation Light Sources Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                 
cl-APC
cl-APC
Crosslinked APC
Crosslinked APC
PB 7.4 650 700000 662
R-PE
R-PE
Lyophilized R-PE
Lyophilized R-PE
PB 7.4 565 1960000 573


These proteins are more stable and have the following features:

Stable
These proteins exhibit long-term storage stabilities.

Highly Sensitive
They exhibit the same high sensitivity and purity as conventional Phycobiliproteins.

Higher Reproducibility
Small lot-to-lot variations allow higher reproducibility.

Flexible Handling
These proteins allow easy adjustment of the concentration and buffer solution. They do not contain any additional chemicals such as sodium azide or amines.

Ready-to-use
No preparation process, including buffer exchange, sample concentration or quality check required. You can start your conjugation instantly after diluting the sample.

 

 

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