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Overview

SETA BioMedicals commercializes fluorescent products for a broad range of applications in the biomedical, pharmaceutical, and environmental research fields. These products are fluorescent detection reagents for biomedical applications - dyes, probes and labels, fluorescent proteins and conjugates.

Our Search Products tool will help you to find a product by type, specific application or spectral characteristics.

Excitation Light Sources Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                   
K1-204
K1-204
SeTau-380-NHS
SeTau-380-NHS
NH2 Water 270 23800 480 56 32.5
K6-208
K6-208
2-DAB
2-DAB
Toluene 464 3600 551 31
K7-1500
K7-1500
Q535
Q535
Toluene 542 15000
K7-547
K7-547
SeTau-405-NHS
SeTau-405-NHS
NH2 PB 7.4 405 13800 518 80 9.3
K7-567
K7-567
SeTau-405-Azide
SeTau-405-Azide
triple-CC PB 7.4 405 13800 518 80 9.3
K8-1342
K8-1342
Seta-670-NHS
Seta-670-NHS
NH2 PB 7.4 667 180000 688 7 0.42
K7-545
K7-545
SeTau-425-NHS
SeTau-425-NHS
NH2 PB 7.4 425 4200 545 39 26.2
K8-1346
K8-1346
Seta-670-Azide
Seta-670-Azide
triple-CC PB 7.4 667 180000 686 7
K8-1351
K8-1351
Square-660-Carboxy
Square-660-Carboxy
PB 7.4 657 182000 676 3 0.27
K8-1352
K8-1352
Square-660-NHS
Square-660-NHS
NH2 PB 7.4 657 182000 676 3 0.27
K8-1357
K8-1357
Square-680-NHS
Square-680-NHS
NH2 MeOH 668 208000 690 10
K8-1407
K8-1407
Square-650-pH-NHS
Square-650-pH-NHS
NH2 PB 5.6 653 135000 671 16 1.17
K8-1500
K8-1500
Square-655
Square-655
PB 7.4 622 177000 644 0.09
K8-1626
K8-1626
Seta-633-di-NHS
Seta-633-di-NHS
NH2 PB 7.4 635 192000 644 12 0.7
K8-1641
K8-1641
Seta-632-Maleimide
Seta-632-Maleimide
SH PB 7.4 633 270000 642 5
K8-1642
K8-1642
Seta-632-NHS
Seta-632-NHS
NH2 PB 7.4 632 280000 641 6
K8-1649
K8-1649
SQ-740-NHS
SQ-740-NHS
NH2 PB 7.4 740 44000 0 0
K8-1663
K8-1663
Seta-633-NHS
Seta-633-NHS
NH2 PB 7.4 633 250000 644 7 0.25
K8-1672
K8-1672
Seta-646-NHS
Seta-646-NHS
NH2 PB 7.4 646 207000 656 10 0.38
K8-1682
K8-1682
Seta-660-di-NHS
Seta-660-di-NHS
NH2 PB 7.4 661 220000 672 11 0.9
K8-1696
K8-1696
Seta-633-Azide
Seta-633-Azide
triple-CC PB 7.4 633 250000 644 7
K8-1902
K8-1902
SQ-565-NHS
SQ-565-NHS
NH2 Water 560 22000 0 0
K8-1922
K8-1922
SQ-780-NHS
SQ-780-NHS
NH2 MeOH 782 115000 0 0
K8-3002
K8-3002 new
Seta-470-NHS
Seta-470-NHS
NH2 water 469 50000 521 1.9
K8-3335
K8-3335
Seta-555-NHS
Seta-555-NHS
NH2 PB 7.4 555 155000 570 7
K8-3345
K8-3345
Seta-555-DBCO
Seta-555-DBCO
N3 PB 7.4 555 155000 570 7
K8-3346
K8-3346
Seta-555-Azide
Seta-555-Azide
triple-CC PB 7.4 555 155000 570 7
K8-5035
K8-5035
Seta-650-NHS
Seta-650-NHS
NH2 PB 7.4 651 200000 671 28
K8-5036
K8-5036
Seta-650-Maleimide
Seta-650-Maleimide
SH PB 7.4 652 200000 672 28
K8-5045
K8-5045
Seta-650-DBCO
Seta-650-DBCO
N3 PB 7.4 653 200000 674 28
K8-5046
K8-5046
Seta-650-Azide
Seta-650-Azide
triple-CC PB 7.4 651 200000 671 28
K8-5075
K8-5075
Seta-680-NHS
Seta-680-NHS
NH2 PB 7.4 678 200000 698 24
K8-7045
K8-7045
Seta-750-DBCO
Seta-750-DBCO
N3 PB 7.4 754 234000 782 21
K8-7046
K8-7046
Seta-750-Azide
Seta-750-Azide
triple-CC PB 7.4 750 200000 779 22
K8-7055
K8-7055
Seta-780-NHS
Seta-780-NHS
NH2 PB 7.4 777 215000 807 1.2
K9-4119
K9-4119 new
SeTau-665-NHS
SeTau-665-NHS
NH2 PB 7.4 664 160000 712 53 3.1
K9-4142
K9-4142
SeTau-647-di-NHS
SeTau-647-di-NHS
NH2 PB 7.4 650 200000 694 65 3.2
K9-4145
K9-4145
SeTau-633-Ethyl-Ester
SeTau-633-Ethyl-Ester
CHCl3 634 88000 683 68
K9-4148
K9-4148
SeTau-647-Maleimide
SeTau-647-Maleimide
SH PB 7.4 648 200000 692 45 3.2
K9-4149
K9-4149
SeTau-647-NHS
SeTau-647-NHS
NH2 PB 7.4 649 200000 695 61 3.2
K9-4169
K9-4169 new
SeTau-670-NHS
SeTau-670-NHS
NH2 PB 7.4 673 275000 694 36 1.6
K8-1388
K8-1388
Seta-700-di-NHS
Seta-700-di-NHS
NH2 PB 7.4 688 180000 704 11
K8-1341
K8-1341
Seta-670-Maleimide
Seta-670-Maleimide
SH PB 7.4 667 180000 688 7
K8-1402
K8-1402
Square-650
Square-650
CHCl3 654 210000 671 53
K8-1671
K8-1671
Seta-646-Maleimide
Seta-646-Maleimide
SH PB 7.4 647 210000 657 8
K8-3010
K8-3010 new
Square-460
Square-460
PB 7.4 467 50000 515 0.9
K4-205
K4-205 new
Seta-375-NHS
Seta-375-NHS
NH2 PB 7.4 372 24500 476 86 3.1
K7-548
K7-548
SeTau-405-Maleimide
SeTau-405-Maleimide
SH PB 7.4 405 13800 518 51 9.1
K8-1405
K8-1405
Square-650-pH-Carboxy
Square-650-pH-Carboxy
NH2 PB 5.6 653 135000 671 16 1.17
K10-6801
K10-6801 new
Seta-PerCP-680
Seta-PerCP-680
PB 7.4 481 380000 676
K10-7801
K10-7801 new
Seta-APC-780
Seta-APC-780
PB 7.4 651 1000000 780
K10-6701
K10-6701 new
Seta-R-PE-670
Seta-R-PE-670
PB 7.4 488 1100000 671
K6-1037
K6-1037
K37
K37
Toluene 434 29500 540 49
K8-7522
K8-7522
Seta-750-NHS
Seta-750-NHS
NH2 PB 7.4 753 230000 780 14
KS-550
KS-550
510–560 nm FP Standard
510–560 nm FP Standard
Water 549 567
KS-660
KS-660
550–670 nm FP Standard
550–670 nm FP Standard
PB 7.4 3 0.27
KS-470
KS-470
436–490 nm FP Standard
436–490 nm FP Standard
PB 7.4 1.9
KS-370
KS-370
330–405 nm FLT Standard
330–405 nm FLT Standard
PB 7.4 86 3.18
KS-405
KS-405
370–420 nm FLT and FP Standard
370–420 nm FLT and FP Standard
Water 405 518 80 9.07
K8-2100
K8-2100 new
YOSeta-1 (1mM solution in DMSO)
YOSeta-1 (1mM solution in DMSO)
dsDNA 492 98800 509 52
K8-3003
K8-3003 new
Seta-470-Maleimide
Seta-470-Maleimide
SH Water 469 50000 521 1.9
K10-0775
K10-0775 new
Seta-R-PE-775
Seta-R-PE-775
PB 7.4 488 1100000 774
K10-7151
K10-7151 new
Seta-APC-715
Seta-APC-715
PB 7.4 651 600000 713
K7-544
K7-544
SeTau-425-Maleimide
SeTau-425-Maleimide
SH PB 7.4 425 4200 545 39 26.2
K4-225
K4-225 new
Seta-385-NHS
Seta-385-NHS
NH2 PB 7.4 384 25000 512 78
K6-5052
K6-5052 new
Seta-405 NHS
Seta-405 NHS
NH2 PB 7.4 401 30000 452
K4-215
K4-215 new
Seta-400-NHS
Seta-400-NHS
NH2 PB 7.4 399 24000 576 34

 

Fluorescent Labels:

Square , Seta , and SeTau dyes are a series of dyes that span the whole visible spectrum and the NIR. They are available with various functionalities: carboxylic acids , as amine-reactive NHS esters for labeling of amino groups on peptides, proteins or amino -modified DNA, as maleimides for coupling to thiol -groups in proteins and thiol -modified oligos and as amino -modified labels (on request).

With a few exceptions all these reagents are available in either 0.5 mg, 1 mg or 5 mg Pack Sizes. Please do inquire about the availability of bulk quantities.

Labeling of Amino-groups:

The following functional groups available for labeling of amino-groups in proteins or amino-modified oligos:

overview
 

Among all these functionalities N-hydroxysuccinimide (NHS) esters are the preferred reactive functionality for labeling of amino-groups due to the formation of highly stable amide bonds with amines. Sulfonyl chlorides also form stable sulfamide bonds but the reaction also generates HCl, which is a not so desirable by-product. In addition sulfonyl chlorides are more difficult to synthesize.

Labelling of Thiol-groups:

 

overview
 

Maleimides are the preferred functionality for labeling of small molecules such as peptide and oligo-nucleotides. Maleimides form bonds with thiols at relatively mild conditions and with reasonable yields. Maleimides are less susceptible to hydrolysis in comparison with NHS esters.

We would like to refer you to our labeling procedures for specific guidance on how to label with NHS-esters or maleimides.

 

Click Chemistry Labels:

click
 

Several of our most sensitive Seta Dyes are now available as azides to be reacted with C≡C-triple bonds in either a Cu(I)- catalized 1,3-dipolar cycloaddition reaction to a triazole (see figure) or as cyclo-octynes for strain-mediated cyclization reactions without the need for a Cu-catalyst. The metal-free reactions have a wider applicability and are compatible with cell-based applications.

Cu-free, strain-mediated cycloaddition
 

 

Seta Dye Series:

The Seta dye series spans the whole visible range and the NIR. These bright and photostable fluorescent labels were selected to cover most of the important wavelengths that are available from common laser sources.

Our newly introduced fluorescent labels Seta-375-NHS and Seta-385-NHS and the popular, blue-diode laser excitable Seta-405-NHS (Pacific BlueTM ) are directed for use with the UV and blue diode laser sources. They combine high quantum yields and photostability and are suitable for STED microscopy.

Seta-470-NHS , and Seta-470-maleimide are highly sensitive viscosity labels with either fluorescence intensity or fluorescence lifetime as the read out parameter (see also product flyer).

Seta 680-NHS , Seta-700-NHS , Seta-750-NHS and Seta-780-NHS are fluorescent labeling reagents that provide a flexible solution for NIR labeling of proteins, peptides or antibodies. These labeling reagents are ideal for in vivo optical imaging and fully compatible with the KODAK In-Vivo Multispectral Imaging System FX, the IVIS Imaging System (Caliper LifeSciences) or the Odyssey Fc Imaging System (Li-COR).

 

 

The lifetime-sensitive Seta labels Seta-633-NHS or Seta-670-NHS exhibit shorter fluorescence lifetimes (FLTs) in aqueous media or when labeled to low molecular weight analytes (drugs or small ligands) but increased brightness and up to ten-times longer FLTs when bound to proteins or oligonucleotides and are unique tracers for FLT-based assays.

 

Squaraine Rotaxanes:

The long-wavelength squaraine-rotaxane series is a new, next generation class of dyes that combines high quantum yields (up to 60%) and high extinction coefficients in the order of 150,000 – 200,000 M –1 cm –1 . These dyes were developed by SETA BioMedicals in order to overcome some of the shortcomings of conventional dyes such as low brightness in aquous solutions, low stability against oxygen species and low photostability.

 

Squaraine rotaxanes are available in reactive, water-soluble formats as carboxy, NHS ester and maleimides but also as hydrophobic probes for imaging applications. More importantly, these rotaxanes are currently considered the best 2P probes and labels on the market combining high sensitivity and extremely high 2PA cross-sections of up to 10,000 GM! 

 

Reactive Lifetime-Labels:

The SeTau lifetime labels have lifetimes in the range between 32 and 9 ns. These dyes are excitable in the UV or blue region and are also useful as polarization probes for the measurement of high MW analytes (small proteins).

 

SeTau-425-NHS for example was used to measure a high molecular-weight analyte such as BSA directly in an FP assay. Importantly the FLTs of these labels do not change upon labeling or binding to proteins or analytes as they are not environment sensitive .

 

Square Dyes:

The Square dyes are more hydrophobic in nature and thus they exhibit a strong environment sensitive behavior and are therefore very suitable to trace binding between a labeled, low-molecular-weight analyte and a larger biomolecules. These dyes are suitable for use with either intensity or lifetime as the read out mode.

More detailed information about specific probes and labels in this series e.g. Square-655 (protein-sensitive fluorescent probe), Square-650-pH-NHS (pH-sensitive label), Square-670-Carboxy (fluorescent probe), and Square-660-NHS or Square-680-NHS (lifetime-sensitive fluorescent labels) are provided in the Applications Section.

 

Zy Dyes:

The water-soluble Zy dye NHS esters and maleimides are structurally identical with the reactive Cy TM dyes (Cy3, Cy5, Cy5.5 and Cy7) of GE Healthcare and are sold at very competitive prices ! Please contact us for price quotations on other derivatives or bulk orders.

 

Fluorescent Probes:

YOSeta-1 is a cell-impermeant fluorescent dye used for staining of double-stranded DNA (same dye as YOYO® 1 ). The unbound YOSeta-1 dye has a very low fluorescence quantum yield but the QY increases several thousand times upon binding to double-stranded DNA (see specs sheet ). The probe is available as a 1 mM solution in DMSO.

Other probes are suitable for the measurement of changes in lipid bilayer hydration of cells, measurement of cholesterol in biological fluids, or are used for proteins analysis . Square probes are probably one of the most sensitive dyes for the quantitative analysis of proteins.

Our new type of extremely photostable and bright squaraine rotaxane probes for cellular imaging are based on the long-wavelength SeTau dye series.

The pH-sensitive probe Square-650-pH is the only probe of its kind which shows lifetime sensitive responses in the physiological pH range.

Our pH-sensitive dyes with a pKa in the alkaline pH range are also available in reactive form for labeling to carrier molecules.

 

 

Phycobiliproteins and Tandem-Conjugates:

Phycobiliproteins hold the record for the largest number of photons emitted before bleaching – they are one of the most sensitive fluorescent reporters known. SETA BioMedicals offers lyophilized versions including new, bright and highly photostable tandems of APC and PE with Seta dyes for use in flow cytometry and other immuofluorescence applications.

 

 

Fluorescence Standards:

Fluorescence lifetime (FLT) standards are used to calibrate or test the performance of time- and frequency-domain instrumentation for luminescence lifetime measurements while fluorescence polarization (FP) standards help to calibrate or test fluorescence instrumentation for polarization measurements. Please visit the relevant links for more information.

Characteristics of Fluorescence Lifetime (FLT) Standards

 Product Number
(Specs Sheet)
Lifetime Standard
(Product Info)
Solvent Lifetime
at 25 o C [ns]
Excitation Range
[nm]
Emission Range
[nm]
KS-370 330-405 nm FLT Standard Phosphate buffer pH 7.4 3.18±0.04 340–410 450–550
  p-Terphenyl Ethanol 1.05 228-320 310-412
  PPO Ethanol 1.4 280-350 330-480
KS-405 370–420 nm FLT and FP Standard Water 9.07±0.03 370–420 490–590
  Anthranilic Acid Ethanol 8.9 290 400
   POPOP        
  Fluorescein, dianion NaOH/Water 4±0.1 400 490-520
  Rhodamine B Ethanol 1.7 500-565 583
  Texas Red Ethanol 4.2 589 615
KS-550 Rose Bengal Water 0.078±0.03 510–560 560–590
KS-650 590–650 nm FLT Standard Phosphate buffer pH 7.4 0.98±0.03 590–650 660–710

 

Dyes for Beads:

The extremely bright fluorescent classification dyes for encoding of microspheres (beads) are used in High-Throughput Screening (HTS) and Multiplexed Analysis Systems.

 

Non-fluorescent Quenchers:

Non-fluorescent quencher molecules for FRET applications , both reactive and non-reactive versions are also available.

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