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Product Number: K8-1665

Product Name:
Seta-635-pH-di-Carboxy
Excitation Light Sources:
635
Medium 1:
PB 7.4
λAb max. [nm]:
640
ε [M–1 cm–1]:
188000
λEm max. [nm]:
656
QY [%]:
33
FLT [ns]:
1.8
Medium 2:
pH 12.0
λAb max. [nm]:
519
λEm max. [nm]:
-
QY [%]:
-
FLT [ns]:
-
Specs Sheet:
Download

Description

  • pH-Sensitive fluorescent probe containing two carboxylic groups and pKa = 10.2.

 

Applications

  • pH-Sensitive applications.
  • Fluorescence lifetime applications — this label exhibits a distinct lifetime change upon binding to a biomolecule.

 

Advantages

  • Perfectly suited for excitation with the 594 and 635-nm diode lasers.
  • Sensitive; high extinction coefficient and high quantum yield.
  • pH-sensitive between pH 9 and pH 11, and pH-insensitive between pH 3 and pH 8.
  • Good aqueous solubility.
  • High photostability; e.g. compared to CypHer 5TM.