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Fluorescence Imaging

Mouse egg cell stained with Square-635 and Square-460

 

Fluorescence imaging utilizes light produced by fluorescent species that are introduced into the specimen of interest. SETA BioMedicals has developed a series of propriatary probes and lables that are extremly bright and photostable fluoresccent dyes for 2P, in-vivo and conventioanal imaging applications.

 

 

 

Recently the group of Prof. Weissleder at the Harvard Medical School has synthesized, characterized and tested three compatible imaging probes based on GLP1R targeting peptides to visualize pancreatic beta cells by intravital microscopy. Of the three conjugates (Alexa-488, Alexa-568 and SeTau-647) presented in their paper the performance of SeTau 647 was by far the best in this series of exendin-4-fluorophore conjugates. They determined that SeTau-647 had the highest signal-to-background ratio and the best in vivo imaging properties.

 

 

 

 

 

 

Excitation Light Sources Characteristics Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Medium λ abs
[nm]
ε
[M–1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                               
K35
K35
K35
K35
Toluene 410 10000 495 60 EtOH 430 13200 552 0.01
K5-3222
K5-3222
Seta-580-NHS
Seta-580-NHS
NH2 PB 7.4 578 90000 602 55 BSA, D/P=1 585 609 15
K6-207
K6-207
3-DAB
3-DAB
Toluene 445 9700 582 43 EtOH 460 8600 665 22 4.0
K6-208
K6-208
2-DAB
2-DAB
Toluene 464 3600 551 31 EtOH 480 2900 670 1.1
K7-1500
K7-1500
Q535
Q535
Toluene 542 15000
K8-1342
K8-1342
Seta-670-NHS
Seta-670-NHS
NH2 PB 7.4 667 180000 688 7 0.42 BSA, D/P=1.5 681 180000 696 27 2.43
K8-1346
K8-1346
Seta-670-Azide
Seta-670-Azide
triple-CC PB 7.4 667 180000 686 7 PB 7.4 667 180000 686 7
K8-1350
K8-1350
Square-670-Carboxy
Square-670-Carboxy
PB 7.4 656 676 1.7 0.29 6g/L BSA 673 693 29
K8-1351
K8-1351
Square-660-Carboxy
Square-660-Carboxy
PB 7.4 657 182000 676 3 0.27 2g/L BSA 679 182000 696 45 3.56
K8-1355
K8-1355
Square-680-Carboxy
Square-680-Carboxy
PB 7.4 654 138000 670 1 1g/L BSA 688 168000 708 62
K8-1357
K8-1357
Square-680-NHS
Square-680-NHS
NH2 MeOH 668 208000 690 10 CHCl3 683 202000 701 47
K8-1384
K8-1384
Seta-700-NHS
Seta-700-NHS
NH2 PB 7.4 687 177000 703 6 IgG D/P=1 705 177000 715 4
K8-1407
K8-1407
Square-650-pH-NHS
Square-650-pH-NHS
NH2 PB 5.6 653 135000 671 16 1.17 IgG, D/P=0.8
pH=2.0
662 135000 677 7 1.52
K8-1440
K8-1440
Square-670
Square-670
pH 7.4 659 677 0.09 1 g/L BSA 683 702 50
K8-1500
K8-1500
Square-655
Square-655
PB 7.4 622 177000 644 0.09 1 g/L BSA 652 188,000 672 80
K8-1626
K8-1626
Seta-633-di-NHS
Seta-633-di-NHS
NH2 PB 7.4 635 192000 644 12 0.7 IgG, D/P=1 639 192000 650 33 3
K8-1641
K8-1641
Seta-632-Maleimide
Seta-632-Maleimide
SH PB 7.4 633 270000 642 5
K8-1642
K8-1642
Seta-632-NHS
Seta-632-NHS
NH2 PB 7.4 632 280000 641 6 IgG, D/P=1 637 647 18
K8-1663
K8-1663
Seta-633-NHS
Seta-633-NHS
NH2 PB 7.4 633 250000 644 7 0.25 IgG D/P=1 637 250000 647 26 1.49
K8-1672
K8-1672
Seta-646-NHS
Seta-646-NHS
NH2 PB 7.4 646 207000 656 10 0.38 IgG, D/P=1 650 207000 661 33
K8-1682
K8-1682
Seta-660-di-NHS
Seta-660-di-NHS
NH2 PB 7.4 661 220000 672 11 0.9 IgG D/P=1 665 220000 675 49
K8-1696
K8-1696
Seta-633-Azide
Seta-633-Azide
triple-CC PB 7.4 633 250000 644 7 PB 7.4 633 250000 644 7
K8-3335
K8-3335
Seta-555-NHS
Seta-555-NHS
NH2 PB 7.4 555 155000 570 7 IgG, D/P=1 556 571 13
K8-3345
K8-3345
Seta-555-DBCO
Seta-555-DBCO
N3 PB 7.4 555 155000 570 7
K8-3346
K8-3346
Seta-555-Azide
Seta-555-Azide
triple-CC PB 7.4 555 155000 570 7
K8-5035
K8-5035
Seta-650-NHS
Seta-650-NHS
NH2 PB 7.4 651 200000 671 28 IgG, D/P=1 654 673 37
K8-5036
K8-5036
Seta-650-Maleimide
Seta-650-Maleimide
SH PB 7.4 652 200000 672 28
K8-5045
K8-5045
Seta-650-DBCO
Seta-650-DBCO
N3 PB 7.4 653 200000 674 28
K8-5046
K8-5046
Seta-650-Azide
Seta-650-Azide
triple-CC PB 7.4 651 200000 671 28
K8-7045
K8-7045
Seta-750-DBCO
Seta-750-DBCO
N3 PB 7.4 754 234000 782 21
K8-7046
K8-7046
Seta-750-Azide
Seta-750-Azide
triple-CC PB 7.4 750 200000 779 22
K8-7055
K8-7055
Seta-780-NHS
Seta-780-NHS
NH2 PB 7.4 777 215000 807 1.2
K9-4142
K9-4142
SeTau-647-di-NHS
SeTau-647-di-NHS
NH2 PB 7.4 650 200000 694 65 3.2 IgG, D/P=0.5 649 200000 691 58 3.2
K9-4145
K9-4145
SeTau-633-Ethyl-Ester
SeTau-633-Ethyl-Ester
CHCl3 634 88000 683 68
K9-4148
K9-4148
SeTau-647-Maleimide
SeTau-647-Maleimide
SH PB 7.4 648 200000 692 45 3.2 PB 7.4 648 200000 692 45 3.2
K9-4149
K9-4149
SeTau-647-NHS
SeTau-647-NHS
NH2 PB 7.4 649 200000 695 61 3.2 IgG, D/P=1 648 200000 694 59 3.3
K9-4150
K9-4150
SeTau-647
SeTau-647
PB 7.4 647 211000 693 59 3.1 PB 7.4 647 211000 693 59 3.1
K8-3402
K8-3402
Zy3-NHS
Zy3-NHS
NH2 PB 7.4 549 150000 564 4 0.3
K8-3403
K8-3403
Zy3-Maleimide
Zy3-Maleimide
SH PB 7.4 549 150000 564 4 0.3
K8-1388
K8-1388
Seta-700-di-NHS
Seta-700-di-NHS
NH2 PB 7.4 688 180000 704 11 IgG D/P=1 696 713 23
K8-1341
K8-1341
Seta-670-Maleimide
Seta-670-Maleimide
SH PB 7.4 667 180000 688 7
K8-1402
K8-1402
Square-650
Square-650
CHCl3 654 210000 671 53
K8-1671
K8-1671
Seta-646-Maleimide
Seta-646-Maleimide
SH PB 7.4 647 210000 657 8 IgG, D/P=1 650 661 31
K8-3010
K8-3010 new
Square-460
Square-460
PB 7.4 467 50000 515 0.9 6g/L BSA 469 512 8.6
K4-205
K4-205 new
Seta-375-NHS
Seta-375-NHS
NH2 PB 7.4 372 24500 476 86 3.1 IgG, D/P=5 372 24500 480 42 3.5
K7-548
K7-548
SeTau-405-Maleimide
SeTau-405-Maleimide
SH PB 7.4 405 13800 518 51 9.1 EtOH 391 15000 498 32 8.5
cl-APC
cl-APC
Crosslinked APC
Crosslinked APC
PB 7.4 650 700000 662
K8-1405
K8-1405
Square-650-pH-Carboxy
Square-650-pH-Carboxy
NH2 PB 5.6 653 135000 671 16 1.17 pH 9.0 535 48000 663 9 0.53
K10-6801
K10-6801 new
Seta-PerCP-680
Seta-PerCP-680
PB 7.4 481 380000 676
K10-7801
K10-7801 new
Seta-APC-780
Seta-APC-780
PB 7.4 651 1000000 780
K10-6701
K10-6701 new
Seta-R-PE-670
Seta-R-PE-670
PB 7.4 488 1100000 671
K8-7522
K8-7522
Seta-750-NHS
Seta-750-NHS
NH2 PB 7.4 753 230000 780 14 IgG D/P=1 756 783 17
K10-0775
K10-0775 new
Seta-R-PE-775
Seta-R-PE-775
PB 7.4 488 1100000 774
K10-7151
K10-7151 new
Seta-APC-715
Seta-APC-715
PB 7.4 651 600000 713
K4-225
K4-225 new
Seta-385-NHS
Seta-385-NHS
NH2 PB 7.4 384 25000 512 78 IgG, D/P=6 387 25000 505 29
K6-5052
K6-5052 new
Seta-405 NHS
Seta-405 NHS
NH2 PB 7.4 401 30000 452 IgG D/P=1 405 553 56
K4-215
K4-215 new
Seta-400-NHS
Seta-400-NHS
NH2 PB 7.4 399 24000 576 34 IgG D/P=1 401 569 19

 

See also NIR Fluorescence Imaging and Fluorescence Lifetime Imaging.

 

 

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