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  • SeTau 647 used in single molecule study of cortical actin nodes

SeTau 647 used in single molecule study of cortical actin nodes

Cortical actin nodes were investigated using super-resolution and single-molecule microscopy techniques with SeTau-647 (K9-4148)-labeled CoA.

 

Movie showing a representative two-color TIRFM observation of Lifeact-mGFP (green) and Lifeact-TM-ACP-SeTau647 (red) at 60 Hz (16.7-ms time resolution) is available here https://doi.org/10.1371/journal.pone.0188778.s007 or online in the citation below.

Excitation Light Sources Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                   
K9-4119
K9-4119 new
SeTau-665-NHS
SeTau-665-NHS
NH2 PB 7.4 664 160000 712 53 3.1
K9-4148
K9-4148
SeTau-647-Maleimide
SeTau-647-Maleimide
SH PB 7.4 648 200000 692 45 3.2
K9-4149
K9-4149
SeTau-647-NHS
SeTau-647-NHS
NH2 PB 7.4 649 200000 695 61 3.2
K9-4169
K9-4169 new
SeTau-670-NHS
SeTau-670-NHS
NH2 PB 7.4 673 275000 694 36 1.6
K9-4179
K9-4179 new
SeTau-680-NHS
SeTau-680-NHS
NH2 PB 7.4 683 215000 705 58 2.9

Citation: Shirai YM, Tsunoyama TA, Hiramoto-Yamaki N, Hirosawa KM, Shibata ACE, Kondo K, et al. (2017) Cortical actin nodes: Their dynamics and recruitment of podosomal proteins as revealed by super-resolution and single-molecule microscopy. PLoS ONE 12(11): e0188778. https://doi.org/10.1371/journal.pone.0188778

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