tech1
$0.00

Product Name: Square-635-Maleimide

Product Number:
K8-1613
Target Group:
SH
Excitation Light Sources:
Medium 1:
pH 7.4
λAb max. [nm]:
636
ε [M–1 cm–1]:
180000
λEm max. [nm]:
649
QY [%]:
8
Specs Sheet:
Download

Description

  • Thiol-reactive, pH-sensitive fluorescent label containing one reactive maleimide group.

 

Applications

  • Covalent labeling of thiol-modified peptides, oligonucleotides, and proteins.
  • Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule.

 

Advantages

  • Perfectly suited for excitation with the 594-nm and 635-nm diode laser.
  • Sensitive; high extinction coefficient and high quantum yield after covalent attachment to proteins.
  • Low non-specific binding. 
  • pH-sensitive between pH 9 and pH 12, and pH-insensitive between pH 3 and pH 9.
  • Good aqueous solubility; this label does not alter the solubility of the protein conjugate. 
  • High photostability; e.g. compared to CypHer 5TM.
  • Low molecular weight  — Squaredyes do not add substantial mass to the conjugates. 
  • Ideal for non-radioactive labeling of thiol-modified peptides, oligonucleotides, and proteins.