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Product Name : Square-635-NHS

Product Number:
K8-1612
Target Group:
NH2
Excitation Light Sources:
Medium 1:
pH 7.4
λAb max. [nm]:
634
ε [M–1 cm–1]:
180000
λEm max. [nm]:
646
QY [%]:
9
Medium 2:
BSA, D/P=1.0
λAb max. [nm]:
649
λEm max. [nm]:
663
QY [%]:
29
Specs Sheet:
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Description

  • Amine-reactive, pH-sensitive fluorescent label containing one reactive NHS-ester group and pKa = 10.7 (free dye).

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides.
  • Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule.

 

Advantages

  • Perfectly suited for excitation with the 594-nm and 635-nm diode laser.
  • Sensitive; high extinction coefficient and high quantum yield up to 40% after covalent attachment to proteins.
  • Low non-specific binding. 
  • pH-sensitive between pH 9 and pH 12, and pH-insensitive between pH 3 and pH 9.
  • Good aqueous solubility; this label does not alter the solubility of the protein conjugate. 
  • High photostability; e.g. compared to CypHer 5TM.
  • Low molecular weight  — Squaredyes do not add substantial mass to the conjugates. 
  • Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides.