Immunofluorescence

Immunofluorescence uses fluorescently labeled antibodies to measure antigens or visualize epitopes of interest on cell surfaces. Primary or secondary antibodies can be utilized for these applications: a primary antibody is used against the antigen of interest directly while the secondary antibody couples to a primary antibody.

SETA BioMedicals offers a large number of superior fluorescent labels for immunofluorescence applications. Seta-labels yield in general fluorescent antibodies that are much brighter and more photostable compared to other dyes. Fluorescent labels and tandems for labeling of CD antibodies are provided in the flow cytometry section. Please visit our website for more information or call us at 217 417 2160.

			Excitation Light Sources												Characteristics
Product Number (Specs Sheet)	Product Name (Product Info)	Target Group	380	405	436	488	532	594	635	650	680	700	750	780	Medium	λ abs [nm]	ε [M ^–1 cm^–1]	λ em [nm]	QY [%]	FLT [ns]	Buy

K1-204 K1-204	SeTau-380-NHS SeTau-380-NHS	NH₂	•	•											Water	270	23,800	480	56	32.5
K7-547 K7-547	SeTau-405-NHS SeTau-405-NHS	NH₂	•	•											PB 7.4	405	13,800	518	80	9.3
K7-545 K7-545	SeTau-425-NHS SeTau-425-NHS	NH₂	•	•	•										PB 7.4	425	4,200	545	39	26.2
K8-1352 K8-1352	Square-660-NHS Square-660-NHS	NH₂	•	•					•	•					PB 7.4	657	182,000	676	3	0.27
K8-1641 K8-1641	Seta-632-Maleimide Seta-632-Maleimide	SH							•						PB 7.4	633	270,000	642	5
K8-1642 K8-1642	Seta-632-NHS Seta-632-NHS	NH₂							•						PB 7.4	632	280,000	641	6
K8-1649 K8-1649	SQ-740-NHS SQ-740-NHS	NH₂								•	•	•	•		PB 7.4	740	44,000		0	0
K8-1663 K8-1663	Seta-633-NHS Seta-633-NHS	NH₂						•	•						PB 7.4	633	250,000	644	7	0.25
K8-1672 K8-1672	Seta-646-NHS Seta-646-NHS	NH₂							•						PB 7.4	646	207,000	656	10	0.38
K8-1682 K8-1682	Seta-660-di-NHS Seta-660-di-NHS	NH₂							•	•					PB 7.4	661	220,000	672	11	0.9
K8-1902 K8-1902	SQ-565-NHS SQ-565-NHS	NH₂					•	•							Water	560	22,000		0	0
K8-1922 K8-1922	SQ-780-NHS SQ-780-NHS	NH₂	•	•	•						•	•	•	•	MeOH	782	115,000		0	0
K8-3335 K8-3335	Seta-555-NHS Seta-555-NHS	NH₂					•								PB 7.4	555	155,000	570	7
K8-5035 K8-5035	Seta-650-NHS Seta-650-NHS	NH₂							•	•					PB 7.4	651	200,000	671	28
K8-5036 K8-5036	Seta-650-Maleimide Seta-650-Maleimide	SH							•	•					PB 7.4	652	200,000	672	28
K8-7046 K8-7046	Seta-750-Azide Seta-750-Azide	triple-CC									•	•	•		PB 7.4	750	200,000	779	22
K8-7055 K8-7055	Seta-780-NHS Seta-780-NHS	NH₂										•	•	•	PB 7.4	777	215,000	807	1.2
K9-4119 K9-4119 ^new	SeTau-665-NHS SeTau-665-NHS	NH₂								•	•	•			PB 7.4	664	160,000	712	53	3.1
K9-4142 K9-4142	SeTau-647-di-NHS SeTau-647-di-NHS	NH₂							•	•					PB 7.4	650	200,000	694	65	3.2
K9-4148 K9-4148	SeTau-647-Maleimide SeTau-647-Maleimide	SH							•	•					PB 7.4	648	200,000	692	45	3.2
K9-4149 K9-4149	SeTau-647-NHS SeTau-647-NHS	NH₂							•	•					PB 7.4	649	200,000	695	61	3.2
K8-3402 K8-3402	Zy3-NHS Zy3-NHS	NH₂					•								PB 7.4	549	150,000	564	4	0.3
K8-5402 K8-5402	Zy5-NHS Zy5-NHS	NH₂							•						PB 7.4	647	250,000	665	27	1
K8-5422 K8-5422	Zy5.5-NHS Zy5.5-NHS	NH₂							•	•	•				PB 7.4	676	190,000	694	23	1
K8-3403 K8-3403	Zy3-Maleimide Zy3-Maleimide	SH					•								PB 7.4	549	150,000	564	4	0.3
K8-5403 K8-5403	Zy5-Maleimide Zy5-Maleimide	SH							•						PB 7.4	647	250,000	665	27	1
K8-5423 K8-5423	Zy5.5-Maleimide Zy5.5-Maleimide	SH							•	•	•				PB 7.4	676	190,000	694	23	1
K8-1388 K8-1388	Seta-700-di-NHS Seta-700-di-NHS	NH₂	•	•						•	•	•			PB 7.4	688	180,000	704	11
K8-1341 K8-1341	Seta-670-Maleimide Seta-670-Maleimide	SH	•	•					•	•					PB 7.4	667	180,000	688	7
K8-1671 K8-1671	Seta-646-Maleimide Seta-646-Maleimide	SH							•						PB 7.4	647	210,000	657	8
K4-205 K4-205 ^new	Seta-375-NHS Seta-375-NHS	NH₂	•												PB 7.4	372	24,500	476	86	3.1
K7-548 K7-548	SeTau-405-Maleimide SeTau-405-Maleimide	SH	•	•											PB 7.4	405	13,800	518	51	9.1
K10-6801 K10-6801 ^new	Seta-PerCP-680 Seta-PerCP-680				•	•	•								PB 7.4	481	380,000	676
K10-7801 K10-7801 ^new	Seta-APC-780 Seta-APC-780								•	•					PB 7.4	651	1,100,000	780
K10-6701 K10-6701 ^new	Seta-R-PE-670 Seta-R-PE-670					•	•								PB 7.4	488	1,100,000	671
K8-7522 K8-7522	Seta-750-NHS Seta-750-NHS	NH₂									•	•	•		PB 7.4	753	230,000	780	14
K8-3003 K8-3003 ^new	Seta-470-Maleimide Seta-470-Maleimide	SH		•	•	•									Water	469	50,000	521	1.9
K8-7402 K8-7402	Zy7-NHS Zy7-NHS	NH₂									•	•	•		PB 7.4	749	200,000	774	13	0.57
K10-0775 K10-0775 ^new	Seta-R-PE-775 Seta-R-PE-775					•	•								PB 7.4	488	1,100,000	774
K10-7051 K10-7051 ^new	Seta-APC-705 Seta-APC-705								•	•					PB 7.4	655	1,100,000	703
K4-225 K4-225 ^new	Seta-385-NHS Seta-385-NHS	NH₂	•												PB 7.4	384	25,000	512	78
K6-5052 K6-5052 ^new	Seta-405 NHS Seta-405 NHS	NH₂	•	•											PB 7.4	401	30,000	452	70
K4-215 K4-215 ^new	Seta-400-NHS Seta-400-NHS	NH₂	•	•											PB 7.4	399	24,000	576	34
K9-4179 K9-4179 ^new	SeTau-680-NHS SeTau-680-NHS	NH₂							•	•	•				PB 7.4	683	295,000	705	58	2.9

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