Product Name: Seta-660-di-NHS
Product Number:
K8-1682 Target Group:
NH2Excitation Light Sources:
Medium 1:
PB 7.4λAb max. [nm]:
661ε [M–1 cm–1]:
220000λEm max. [nm]:
672QY [%]:
11FLT [ns]:
0.9Medium 2:
IgG D/P=1λAb max. [nm]:
665ε [M–1 cm–1]:
220000λEm max. [nm]:
675QY [%]:
49Specs Sheet:
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Description
- Extremely bright, hydrophilic, amine-reactive label containing two NHS-ester groups. QYs of up to 50% when covalently labeled to IgG.
Applications
- Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
- Fluorescence intensity and fluorescence polarization-based applications
- Resonance Energy Transfer (RET)
- Flow Cytometry
- Immunofluorescence
- Gene Expression
- Homogeneous Assays
- Microarrays
Advantages
- Perfectly suited for excitation with the 635-nm and 650-nm diode lasers
- Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules
- Brighter replacement for Alexa 660 (QY = 37%, ε = 132,000 M–1cm–1). Seta-660: QY = 49%, ε = 220,000 M–1cm–1
- pH-insensitive between pH 3 and pH 10
- Good aqueous solubility; this label does not alter the solubility of the bioconjugate
- High photostability; e.g. compared to fluorescein, Cy5 and Alexa 647 or Alexa 660
- Low molecular weight — Seta dyes do not add substantial mass to the conjugates
- Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides and amino-modified lipids
