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Immunofluorescence
The next-generation squaraine rotaxane dye SeTau 647 (K9-4149) is currently the brightest label on the market for the Kr-ion laser line
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| Quantum yields of IgG-conjugates of SeTau 647, Alexa 647 and Cy5 at various dye-to-protein ratios in phosphat buffer (pH 7.4) |
Plasmonics
Seta dyes show much higher signal enhancement (SE) on silver surfaces (SIFs): SE > 15 compared to Cy5, Alexa 647 or phycobillproteins (PE, APC) - SE ~ 6 - 7. |
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The squaraine rotaxane SeTau 665 (K9-4113) shows record signal-enhancement on SIFs:SE ~ 75 for bulk measurements and SE ~ 400 for single-molecule measurements
2-Photon FRET
2-Photon FRET pairs and SETA FRET software for
2-photon FRET microscopy applications
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