Phycobiliproteins are water-soluble, light-harvesting proteins that are highly fluorescent due to the incorporation of tetrapyrrole chromophores. They are isolated from cyanobacteria and eukaryotic algae. Phycobiliproteins hold the record for the largest number of photons emitted before bleaching – they are one of the most sensitive fluorescent reporters known. They combine extremely high extinction coefficients and quantum yields close to unity (see below). Because of their high sensitivity, phycobiliprotein-labeled antibodies and other molecules are used in fluorescence applications such as flow cytometry and immuno-blotting/immuno-staining. They are also used as energy tranfer donors in tandem-constructs for multi-color analysis in flow cytometry.
SETA BioMedicals offers lyophilized phycobiliproteins.
Excitation Light Sources
These proteins are more stable and have the following features:
These proteins exhibit long-term storage stabilities.
They exhibit the same high sensitivity and purity as conventional Phycobiliproteins.
Small lot-to-lot variations allow higher reproducibility.
These proteins allow easy adjustment of the concentration and buffer solution. They do not contain any additional chemicals such as sodium azide or amines.
No preparation process, including buffer exchange, sample concentration or quality check required. You can start your conjugation instantly after diluting the sample.