Product Name: Seta-633-NHS

Product Number:

K8-1663

Target Group:

NH₂

Excitation Light Sources:

Medium 1:

PB 7.4

λ_Ab max. [nm]:

633

ε [M^–1cm^–1]:

250000

λ_Em max. [nm]:

644

QY [%]:

FLT [ns]:

0.25

Medium 2:

IgG D/P=1

λ_Ab max. [nm]:

637

ε [M^–1cm^–1]:

250000

λ_Em max. [nm]:

647

QY [%]:

FLT [ns]:

1.49

Medium 3:

BSA D/P=1

λ_Ab max. [nm]:

646

λ_Em max. [nm]:

656

QY [%]:

Specs Sheet:

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Description

Highly hydrophilic, amine-reactive label containing one NHS-ester group. Brighter (ε = 250,000 M^–1cm^–1, QY = 26% (IgG, D/P =1)) and more photostable replacement for Alexa 633.

Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
Fluorescence intensity and fluorescence polarization-based applications
Resonance Energy Transfer (RET)
Flow Cytometry
Immunofluorescence
Gene Expression
Homogeneous Assays
Microarrays

Perfectly suited for excitation with the 594, 633 or 635 nm diode lasers
Sensitive; high extinction coefficients and quantum yields highly increase after covalent attachment to biomolecules
pH-insensitive between pH 3 and pH 10
Good aqueous solubility; this label does not alter the solubility of the bioconjugate
High photostability; e.g. compared to fluorescein, Cy5^TM or Alexa Fluor^TM 647
Low molecular weight — Seta dyes do not add substantial mass to the conjugates
Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides and amino-modified lipids