Product Name: Seta-375-NHS

Product Number:
K4-205
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
372
ε [M–1 cm–1]:
24500
λEm max. [nm]:
476
QY [%]:
86
FLT [ns]:
3.1
Medium 2:
IgG, D/P=5
λAb max. [nm]:
372
ε [M–1 cm–1]:
24500
λEm max. [nm]:
480
QY [%]:
42
FLT [ns]:
3.5
Specs Sheet:
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Description

  • Extremely bright, positively charged, water-soluble amine-reactive label containing one NHS-ester group

 

Applications

  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
  • Resonance Energy Transfer (RET)
  • Flow Cytometry
  • Immunofluorescence
  • Gene Expression
  • Homogeneous Assays
  • Assessment of protein structure

 

Advantages

  • Perfectly suited for excitation with 330, 375 and 380-nm diode lasers
  • Large Stokes' shift of about 100 nm
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides